Mitochondrial DNA sequence variation among triatomine vectors of Chagas' disease

Daniel F. Lyman, Fernando A. Monteiro, Ananias A. Escalante, Celia Cordon-Rosales, Dawn M. Wesson, Jean Pierre Dujardin, Charles B. Beard

Research output: Contribution to journalArticle

106 Citations (Scopus)

Abstract

Kissing bugs or triatomines (Reduviidae: Triatominae) are vectors of the Chagas' disease agent Trypanosoma cruzi. There is a current need for more sensitive tools for use in discrimination of different bug populations and species, thus allowing a better understanding of these insects as it relates to disease transmission and control. In a preliminary analysis of the mitochondrial large subunit ribosomal RNA (mtlsurRNA) and cytochrome B (mtCytB) genes, we used DNA sequencing to study species identification and phylogeny. In both examined gene regions, about 46% of nucleotide positions exhibited polymorphism. The examined region of mtCytB appears to have evolved more rapidly than the examined region of mtlsurRNA. Phylogenetic analysis of both gene fragments in the examined species produced similar results that were generally consistent with the accepted taxonomy of the subfamily. The two major tribes, Rhodniini and Triatomini, were supported, along with additional clades that corresponded to accepted species complexes within the Rhodnius and Triatoma genera. The one chief exception was that Psammolestes coreodes sorted into the Rhodnius prolixus-robustus-neglectus clade, with bootsrap values of 99% and 81%, respectively, for the mtlsurRNA and mtCytB fragments. All of the individual species examined could be distinguished at both genetic loci.

Original languageEnglish (US)
Pages (from-to)377-386
Number of pages10
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume60
Issue number3
StatePublished - 1999
Externally publishedYes

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Triatominae
Reduviidae
Ribosomal RNA
Chagas Disease
Cytochromes
Mitochondrial DNA
Rhodnius
Triatoma
Genes
Genetic Loci
Trypanosoma cruzi
Phylogeny
DNA Sequence Analysis
Insects
Nucleotides
Population

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Lyman, D. F., Monteiro, F. A., Escalante, A. A., Cordon-Rosales, C., Wesson, D. M., Dujardin, J. P., & Beard, C. B. (1999). Mitochondrial DNA sequence variation among triatomine vectors of Chagas' disease. American Journal of Tropical Medicine and Hygiene, 60(3), 377-386.

Mitochondrial DNA sequence variation among triatomine vectors of Chagas' disease. / Lyman, Daniel F.; Monteiro, Fernando A.; Escalante, Ananias A.; Cordon-Rosales, Celia; Wesson, Dawn M.; Dujardin, Jean Pierre; Beard, Charles B.

In: American Journal of Tropical Medicine and Hygiene, Vol. 60, No. 3, 1999, p. 377-386.

Research output: Contribution to journalArticle

Lyman, DF, Monteiro, FA, Escalante, AA, Cordon-Rosales, C, Wesson, DM, Dujardin, JP & Beard, CB 1999, 'Mitochondrial DNA sequence variation among triatomine vectors of Chagas' disease', American Journal of Tropical Medicine and Hygiene, vol. 60, no. 3, pp. 377-386.
Lyman DF, Monteiro FA, Escalante AA, Cordon-Rosales C, Wesson DM, Dujardin JP et al. Mitochondrial DNA sequence variation among triatomine vectors of Chagas' disease. American Journal of Tropical Medicine and Hygiene. 1999;60(3):377-386.
Lyman, Daniel F. ; Monteiro, Fernando A. ; Escalante, Ananias A. ; Cordon-Rosales, Celia ; Wesson, Dawn M. ; Dujardin, Jean Pierre ; Beard, Charles B. / Mitochondrial DNA sequence variation among triatomine vectors of Chagas' disease. In: American Journal of Tropical Medicine and Hygiene. 1999 ; Vol. 60, No. 3. pp. 377-386.
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