Misfolded proteins impose a dosage-dependent fitness cost and trigger a cytosolic unfolded protein response in yeast

Kerry A. Geiler-Samerotte, Michael F. Dion, Bogdan A. Budnik, Stephanie M. Wang, Daniel L. Hartl, D. Allan Drummond

Research output: Contribution to journalArticle

153 Citations (Scopus)

Abstract

Evolving lineages face a constant intracellular threat: most new coding sequence mutations destabilize the folding of the encoded protein. Misfolded proteins form insoluble aggregates and are hypothesized to be intrinsically cytotoxic. Here, we experimentally isolate a fitness cost caused by toxicity of misfolded proteins. We exclude other costs of protein misfolding, such as loss of functional protein or attenuation of growth-limiting protein synthesis resources, by comparing growth rates of budding yeast expressing folded or misfolded variants of a gratuitous protein, YFP, at equal levels. We quantify a fitness cost that increases with misfolded protein abundance, up to as much as a 3.2% growth rate reduction when misfolded YFP represents less than 0.1% of total cellular protein. Comparable experiments on variants of the yeast gene orotidine-5′-phosphate decarboxylase (URA3) produce similar results. Quantitative proteomic measurements reveal that, within the cell, misfolded YFP induces coordinated synthesis of interacting cytosolic chaperone proteins in the absence of a wider stress response, providing evidence for an evolved modular response to misfolded proteins in the cytosol. These results underscore the distinct and evolutionarily relevant molecular threat of protein misfolding, independent of protein function. Assuming that most misfolded proteins impose similar costs, yeast cells express almost all proteins at steady-state levels sufficient to expose their encoding genes to selection against misfolding, lending credibility to the recent suggestion that such selection imposes a global constraint on molecular evolution.

Original languageEnglish (US)
Pages (from-to)680-685
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume108
Issue number2
DOIs
StatePublished - Jan 11 2011
Externally publishedYes

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Unfolded Protein Response
Yeasts
Costs and Cost Analysis
Proteins
Orotidine-5'-Phosphate Decarboxylase
Growth
Saccharomycetales
Molecular Evolution
Protein Folding
Cytosol
Proteomics

Keywords

  • Evolutionary rate
  • Heat shock
  • Proteomics
  • Stability

ASJC Scopus subject areas

  • General

Cite this

Misfolded proteins impose a dosage-dependent fitness cost and trigger a cytosolic unfolded protein response in yeast. / Geiler-Samerotte, Kerry A.; Dion, Michael F.; Budnik, Bogdan A.; Wang, Stephanie M.; Hartl, Daniel L.; Drummond, D. Allan.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, No. 2, 11.01.2011, p. 680-685.

Research output: Contribution to journalArticle

Geiler-Samerotte, Kerry A. ; Dion, Michael F. ; Budnik, Bogdan A. ; Wang, Stephanie M. ; Hartl, Daniel L. ; Drummond, D. Allan. / Misfolded proteins impose a dosage-dependent fitness cost and trigger a cytosolic unfolded protein response in yeast. In: Proceedings of the National Academy of Sciences of the United States of America. 2011 ; Vol. 108, No. 2. pp. 680-685.
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