Miniaturized silicon apertures for lipid bilayer reconstitution experiments

Michael Goryll, Nipun Chaplot

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Scopus citations

Abstract

Ion channels reconstituted into lipid bilayer membranes can be used as a very sensitive and selective platform for high-throughput drug screening applications. In order to employ suspended lipid bilayer membranes for these experiments in form of a "lab-on-a-chip" configuration, a robust and affordable platform is required. In our study, we investigated the feasibility of hosting lipid bilayer membranes across micron-size apertures ranging from 5 μm - 50 μm in silicon. On these substrates, lipid bilayers were formed and characterized concerning their seal resistance, capacitance and breakdown voltage. Seal resistance values of up to 60 GΩ could be achieved repeatedly on these substrates.

Original languageEnglish (US)
Title of host publicationMaterials and Strategies for Lab-on-a-Chip - Biological Analysis, Cell-Material Interfaces and Fluidic Assembly of Nanostructures
Pages79-84
Number of pages6
StatePublished - Dec 1 2009
Event2009 MRS Spring Meeting - San Francisco, CA, United States
Duration: Apr 13 2009Apr 17 2009

Publication series

NameMaterials Research Society Symposium Proceedings
Volume1191
ISSN (Print)0272-9172

Other

Other2009 MRS Spring Meeting
CountryUnited States
CitySan Francisco, CA
Period4/13/094/17/09

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ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

Cite this

Goryll, M., & Chaplot, N. (2009). Miniaturized silicon apertures for lipid bilayer reconstitution experiments. In Materials and Strategies for Lab-on-a-Chip - Biological Analysis, Cell-Material Interfaces and Fluidic Assembly of Nanostructures (pp. 79-84). (Materials Research Society Symposium Proceedings; Vol. 1191).