Miniaturized HPLC column with nano-pore beads for protein separation on flexible medical implants

Yongmo Yang; Junseok Chae

Miniaturized HPLC column with nano-pore beads for protein separation on flexible medical implants

Yongmo Yang, Junseok Chae

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

This paper reports a miniaturized HPLC (High Performance Liquid Chromatography) column packed with nano-pore beads for flexible medical implants applications. The all-flexible-polymer column capable of integrating with micro/nano-devices on a chip separates biological entities to relax stringent requirements on subsequent detectors and to enable parallel detection in a detector array. The fabricated device has successfully separated a protein mixture using nano-pore beads in a miniaturized column. According to HPLC analysis, the intensity ratio of large to small protein varies significantly, more than a factor of 300, over sample collecting time, ∼ 12mins, suggesting very high separation performance.

Original language	English (US)
Title of host publication	Proceedings - CIS Workshops 2007, 2007 International Conference on Computational Intelligence and Security Workshops, CISW 2007
Pages	421-424
Number of pages	4
State	Published - Dec 1 2007
Event	20th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2007 - Kobe, Japan Duration: Jan 21 2007 → Jan 25 2007

Publication series

Name	Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)	1084-6999

Other

Other	20th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2007
Country/Territory	Japan
City	Kobe
Period	1/21/07 → 1/25/07

Keywords

HPLC column
Medical implant
Protein separation
Size Exclusion Chromatography (SEC)

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Mechanical Engineering
Electrical and Electronic Engineering

Cite this

Yang, Y., & Chae, J. (2007). Miniaturized HPLC column with nano-pore beads for protein separation on flexible medical implants. In Proceedings - CIS Workshops 2007, 2007 International Conference on Computational Intelligence and Security Workshops, CISW 2007 (pp. 421-424). Article 4432980 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

Miniaturized HPLC column with nano-pore beads for protein separation on flexible medical implants. / Yang, Yongmo; Chae, Junseok.
Proceedings - CIS Workshops 2007, 2007 International Conference on Computational Intelligence and Security Workshops, CISW 2007. 2007. p. 421-424 4432980 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Yang, Y & Chae, J 2007, Miniaturized HPLC column with nano-pore beads for protein separation on flexible medical implants. in Proceedings - CIS Workshops 2007, 2007 International Conference on Computational Intelligence and Security Workshops, CISW 2007., 4432980, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), pp. 421-424, 20th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2007, Kobe, Japan, 1/21/07.

Yang, Yongmo ; Chae, Junseok. / Miniaturized HPLC column with nano-pore beads for protein separation on flexible medical implants. Proceedings - CIS Workshops 2007, 2007 International Conference on Computational Intelligence and Security Workshops, CISW 2007. 2007. pp. 421-424 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

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abstract = "This paper reports a miniaturized HPLC (High Performance Liquid Chromatography) column packed with nano-pore beads for flexible medical implants applications. The all-flexible-polymer column capable of integrating with micro/nano-devices on a chip separates biological entities to relax stringent requirements on subsequent detectors and to enable parallel detection in a detector array. The fabricated device has successfully separated a protein mixture using nano-pore beads in a miniaturized column. According to HPLC analysis, the intensity ratio of large to small protein varies significantly, more than a factor of 300, over sample collecting time, ∼ 12mins, suggesting very high separation performance.",

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AB - This paper reports a miniaturized HPLC (High Performance Liquid Chromatography) column packed with nano-pore beads for flexible medical implants applications. The all-flexible-polymer column capable of integrating with micro/nano-devices on a chip separates biological entities to relax stringent requirements on subsequent detectors and to enable parallel detection in a detector array. The fabricated device has successfully separated a protein mixture using nano-pore beads in a miniaturized column. According to HPLC analysis, the intensity ratio of large to small protein varies significantly, more than a factor of 300, over sample collecting time, ∼ 12mins, suggesting very high separation performance.

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Miniaturized HPLC column with nano-pore beads for protein separation on flexible medical implants

Abstract

Publication series

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Keywords

ASJC Scopus subject areas

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