Miniature structured illumination microscope for in vivo 3D imaging of brain structures with optical sectioning

Omkar D. Supekar, Andrew Sias, Sean R. Hansen, Gabriel Martinez, Graham C. Peet, Xiaoyu Peng, Victor M. Bright, Ethan G. Hughes, Diego Restrepo, Douglas P. Shepherd, Cristin G. Welle, Juliet T. Gopinath, Emily A. Gibson

Research output: Contribution to journalArticlepeer-review

Abstract

We present a high-resolution miniature, light-weight fluorescence microscope with electrowetting lens and onboard CMOS for high resolution volumetric imaging and structured illumination for rejection of out-of-focus and scattered light. The miniature microscope (SIMscope3D) delivers structured light using a coherent fiber bundle to obtain optical sectioning with an axial resolution of 18 µm. Volumetric imaging of eGFP labeled cells in fixed mouse brain tissue at depths up to 260 µm is demonstrated. The functionality of SIMscope3D to provide background free 3D imaging is shown by recording time series of microglia dynamics in awake mice at depths up to 120 µm in the brain.

Original languageEnglish (US)
Pages (from-to)2530-2541
Number of pages12
JournalBiomedical Optics Express
Volume13
Issue number4
DOIs
StatePublished - Apr 1 2022
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

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