Miniature isothermal amplification reactor with an integrated optical detection unit

Szu Yuan Lee, Lung Jieh Yang, Deirdre R. Meldrum, Chun Nan Lee, Chii Wann Lin

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

In an era of rapid population ageing, “point-ofcare” will be a general trend. It is indispensable for a medical device to possess the characteristics of portability, easy handling and convenient result-reading. In this study, we combine the nucleic acid amplification method with precise processes to develop a miniature biosensor. The loop-mediated amplification method (LAMP) has been used to amplify DNA with high specificity and efficiency under isothermal condition (65ºC) within one hour. The microchip consisted of poly (dimethylsiloxane) (PDMS) and mutilayer thin-film structure has temperature control set and optical detection unit to create appropriate working environment for LAMP reaction. With the increasing of LAMP product, we can detect the reaction by-product sharply by surface plasmon resonance (SPR) and optical interference. Furthermore, we would like to take the partial gene of hepatitis B virus (HBV) as a detection target in our experiment. At present, we have accomplished the specificity and sensitivity test of LAMP reaction. The sensitivity of HBV LAMP reaction determined by agarose gel electrophoresis is 150 copies/tube. Therefore, we are going to develop a miniature isothermal amplification system with an integrated optical detection unit to provide a portable, label-free and real-time monitoring biochip.

Original languageEnglish (US)
Title of host publicationIFMBE Proceedings
EditorsSun I. Kim, Tae Suk Suh
PublisherSpringer Verlag
Pages648-650
Number of pages3
Edition1
ISBN (Print)9783540368397
DOIs
StatePublished - Jan 1 2007
Externally publishedYes
Event10th World Congress on Medical Physics and Biomedical Engineering, WC 2006 - Seoul, Korea, Republic of
Duration: Aug 27 2006Sep 1 2006

Publication series

NameIFMBE Proceedings
Number1
Volume14
ISSN (Print)1680-0737
ISSN (Electronic)1433-9277

Other

Other10th World Congress on Medical Physics and Biomedical Engineering, WC 2006
CountryKorea, Republic of
CitySeoul
Period8/27/069/1/06

Keywords

  • Isothermal
  • LAMP
  • MEMS
  • Micro-reactor
  • Plamonics
  • Polymerase chain reaction

ASJC Scopus subject areas

  • Bioengineering
  • Biomedical Engineering

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  • Cite this

    Lee, S. Y., Yang, L. J., Meldrum, D. R., Lee, C. N., & Lin, C. W. (2007). Miniature isothermal amplification reactor with an integrated optical detection unit. In S. I. Kim, & T. S. Suh (Eds.), IFMBE Proceedings (1 ed., pp. 648-650). (IFMBE Proceedings; Vol. 14, No. 1). Springer Verlag. https://doi.org/10.1007/978-3-540-36841-0_150