Milestones in electron crystallography

Ludovic Renault; Hui Ting Chou; Po Lin Chiu; Rena M. Hill; Xiangyan Zeng; Bryant Gipson; Zi Yan Zhang; Anchi Cheng; Vinzenz Unger; Henning Stahlberg

doi:10.1007/s10822-006-9075-x

Milestones in electron crystallography

Ludovic Renault, Hui Ting Chou, Po Lin Chiu, Rena M. Hill, Xiangyan Zeng, Bryant Gipson, Zi Yan Zhang, Anchi Cheng, Vinzenz Unger, Henning Stahlberg

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

Electron crystallography determines the structure of membrane embedded proteins in the two-dimensionally crystallized state by cryo-transmission electron microscopy imaging and computer structure reconstruction. Milestones on the path to the structure are high-level expression, purification of functional protein, reconstitution into two-dimensional lipid membrane crystals, high-resolution imaging, and structure determination by computer image processing. Here we review the current state of these methods. We also created an Internet information exchange platform for electron crystallography, where guidelines for imaging and data processing method are maintained. The server (http://2dx.org) provides the electron crystallography community with a central information exchange platform, which is structured in blog and Wiki form, allowing visitors to add comments or discussions. It currently offers a detailed step-by-step introduction to image processing with the MRC software program. The server is also a repository for the 2dx software package, a user-friendly image processing system for 2D membrane protein crystals.

Original language	English (US)
Pages (from-to)	519-527
Number of pages	9
Journal	Journal of Computer-Aided Molecular Design
Volume	20
Issue number	7-8
DOIs	https://doi.org/10.1007/s10822-006-9075-x
State	Published - Aug 2006
Externally published	Yes

Keywords

2D crystallization
2dx
Electron crystallography
Electron microscopy
Image processing
Membrane proteins

ASJC Scopus subject areas

Drug Discovery
Computer Science Applications
Physical and Theoretical Chemistry

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10.1007/s10822-006-9075-x

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title = "Milestones in electron crystallography",

abstract = "Electron crystallography determines the structure of membrane embedded proteins in the two-dimensionally crystallized state by cryo-transmission electron microscopy imaging and computer structure reconstruction. Milestones on the path to the structure are high-level expression, purification of functional protein, reconstitution into two-dimensional lipid membrane crystals, high-resolution imaging, and structure determination by computer image processing. Here we review the current state of these methods. We also created an Internet information exchange platform for electron crystallography, where guidelines for imaging and data processing method are maintained. The server (http://2dx.org) provides the electron crystallography community with a central information exchange platform, which is structured in blog and Wiki form, allowing visitors to add comments or discussions. It currently offers a detailed step-by-step introduction to image processing with the MRC software program. The server is also a repository for the 2dx software package, a user-friendly image processing system for 2D membrane protein crystals.",

keywords = "2D crystallization, 2dx, Electron crystallography, Electron microscopy, Image processing, Membrane proteins",

author = "Ludovic Renault and Chou, {Hui Ting} and Chiu, {Po Lin} and Hill, {Rena M.} and Xiangyan Zeng and Bryant Gipson and Zhang, {Zi Yan} and Anchi Cheng and Vinzenz Unger and Henning Stahlberg",

note = "Funding Information: Acknowledgements This work was in part supported by the NSF, grant number MCB-0447860 and by the NIH, grant number U54-GM074929. V. Unger is supported by NIH grants GM66145 and GM071590. We thank Y. Fujiyoshi and J. Fethi{\`e}re for providing material for Fig. 1.",

year = "2006",

month = aug,

doi = "10.1007/s10822-006-9075-x",

language = "English (US)",

volume = "20",

pages = "519--527",

journal = "Journal of Computer-Aided Molecular Design",

issn = "0920-654X",

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T1 - Milestones in electron crystallography

AU - Renault, Ludovic

AU - Chou, Hui Ting

AU - Chiu, Po Lin

AU - Hill, Rena M.

AU - Zeng, Xiangyan

AU - Gipson, Bryant

AU - Zhang, Zi Yan

AU - Cheng, Anchi

AU - Unger, Vinzenz

AU - Stahlberg, Henning

N1 - Funding Information: Acknowledgements This work was in part supported by the NSF, grant number MCB-0447860 and by the NIH, grant number U54-GM074929. V. Unger is supported by NIH grants GM66145 and GM071590. We thank Y. Fujiyoshi and J. Fethière for providing material for Fig. 1.

PY - 2006/8

Y1 - 2006/8

N2 - Electron crystallography determines the structure of membrane embedded proteins in the two-dimensionally crystallized state by cryo-transmission electron microscopy imaging and computer structure reconstruction. Milestones on the path to the structure are high-level expression, purification of functional protein, reconstitution into two-dimensional lipid membrane crystals, high-resolution imaging, and structure determination by computer image processing. Here we review the current state of these methods. We also created an Internet information exchange platform for electron crystallography, where guidelines for imaging and data processing method are maintained. The server (http://2dx.org) provides the electron crystallography community with a central information exchange platform, which is structured in blog and Wiki form, allowing visitors to add comments or discussions. It currently offers a detailed step-by-step introduction to image processing with the MRC software program. The server is also a repository for the 2dx software package, a user-friendly image processing system for 2D membrane protein crystals.

AB - Electron crystallography determines the structure of membrane embedded proteins in the two-dimensionally crystallized state by cryo-transmission electron microscopy imaging and computer structure reconstruction. Milestones on the path to the structure are high-level expression, purification of functional protein, reconstitution into two-dimensional lipid membrane crystals, high-resolution imaging, and structure determination by computer image processing. Here we review the current state of these methods. We also created an Internet information exchange platform for electron crystallography, where guidelines for imaging and data processing method are maintained. The server (http://2dx.org) provides the electron crystallography community with a central information exchange platform, which is structured in blog and Wiki form, allowing visitors to add comments or discussions. It currently offers a detailed step-by-step introduction to image processing with the MRC software program. The server is also a repository for the 2dx software package, a user-friendly image processing system for 2D membrane protein crystals.

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KW - Electron crystallography

KW - Electron microscopy

KW - Image processing

KW - Membrane proteins

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Milestones in electron crystallography

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Keywords

ASJC Scopus subject areas

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