Milestones in electron crystallography

Ludovic Renault, Hui Ting Chou, Po Lin Chiu, Rena M. Hill, Xiangyan Zeng, Bryant Gipson, Zi Yan Zhang, Anchi Cheng, Vinzenz Unger, Henning Stahlberg

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

Electron crystallography determines the structure of membrane embedded proteins in the two-dimensionally crystallized state by cryo-transmission electron microscopy imaging and computer structure reconstruction. Milestones on the path to the structure are high-level expression, purification of functional protein, reconstitution into two-dimensional lipid membrane crystals, high-resolution imaging, and structure determination by computer image processing. Here we review the current state of these methods. We also created an Internet information exchange platform for electron crystallography, where guidelines for imaging and data processing method are maintained. The server (http://2dx.org) provides the electron crystallography community with a central information exchange platform, which is structured in blog and Wiki form, allowing visitors to add comments or discussions. It currently offers a detailed step-by-step introduction to image processing with the MRC software program. The server is also a repository for the 2dx software package, a user-friendly image processing system for 2D membrane protein crystals.

Original languageEnglish (US)
Pages (from-to)519-527
Number of pages9
JournalJournal of Computer-Aided Molecular Design
Volume20
Issue number7-8
DOIs
StatePublished - Aug 2006

Keywords

  • 2D crystallization
  • 2dx
  • Electron crystallography
  • Electron microscopy
  • Image processing
  • Membrane proteins

ASJC Scopus subject areas

  • Drug Discovery
  • Computer Science Applications
  • Physical and Theoretical Chemistry

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