Microtubule dynamics and the role of molecular motors in Neurospora crassa

Maho Uchida, Rosa R. Mouriño-Pérez, Michael Freitag, Salomon Bartnicki-García, Robert Roberson

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Live-cell imaging methods were used to study microtubule dynamics in the apical regions of leading hyphae and germ tubes of Neurospora crassa expressing β-tubulin-GFP. Microtubule polymerization rates in hyphae of N. crassa were much faster than those previously reported in any other eukaryotic organism. In order to address the roles of motor proteins in microtubule dynamic instability in N. crassa, the microtubule-motor mutant strains, Δnkin and ro-1, were examined. Polymerization and depolymerization rates in leading hyphae of these strains were reduced by one half relative to the wild type. Furthermore, microtubules in germ tubes of wild type and microtubule-motor mutants exhibited similar dynamic characteristics as those in hyphae of mutant strains. Small microtubule fragments exhibiting anterograde and retrograde motility were present in leading hyphae of all strains and germ tubes of wild-type strains. Our data suggest that microtubule motors play important roles in regulating microtubule dynamic instability in leading hyphae but not in germ tubes.

Original languageEnglish (US)
Pages (from-to)683-692
Number of pages10
JournalFungal Genetics and Biology
Volume45
Issue number5
DOIs
StatePublished - May 2008

Fingerprint

Neurospora crassa
Molecular Dynamics Simulation
Microtubules
Hyphae
Polymerization
Microtubule Proteins
Tubulin

Keywords

  • Dynamic instability
  • Dynein
  • Green-fluorescent protein
  • Kinesin
  • Live-cell imaging

ASJC Scopus subject areas

  • Genetics
  • Microbiology

Cite this

Microtubule dynamics and the role of molecular motors in Neurospora crassa. / Uchida, Maho; Mouriño-Pérez, Rosa R.; Freitag, Michael; Bartnicki-García, Salomon; Roberson, Robert.

In: Fungal Genetics and Biology, Vol. 45, No. 5, 05.2008, p. 683-692.

Research output: Contribution to journalArticle

Uchida, M, Mouriño-Pérez, RR, Freitag, M, Bartnicki-García, S & Roberson, R 2008, 'Microtubule dynamics and the role of molecular motors in Neurospora crassa', Fungal Genetics and Biology, vol. 45, no. 5, pp. 683-692. https://doi.org/10.1016/j.fgb.2007.10.013
Uchida, Maho ; Mouriño-Pérez, Rosa R. ; Freitag, Michael ; Bartnicki-García, Salomon ; Roberson, Robert. / Microtubule dynamics and the role of molecular motors in Neurospora crassa. In: Fungal Genetics and Biology. 2008 ; Vol. 45, No. 5. pp. 683-692.
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