Microtubule dynamics and organization during hyphal growth and branching in Neurospora crassa

Rosa R. Mouriño-Pérez, Robert Roberson, Salomon Bartnicki-García

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

By confocal microscopy, we analyzed microtubule (Mt) behavior during hyphal growth and branching in a Neurospora crassa strain whose Mts had been tagged with GFP. Images were assembled spatially and temporally to better understand the 3-D organization of the microtubular cytoskeleton and a clearer view of its dynamics. Cytoplasmic Mts were mainly arranged longitudinally along the hyphal tube. Straight segments were rare; most Mts showed a distinct helical curvature with a long pitch and a tendency to intertwine with one another to form a loosely braided network throughout the cytoplasm. This study revealed that the microtubular cytoskeleton of a hypha advances as a unit, i.e., as the cell elongates, it moves forward by bulk flow. Nuclei appeared trapped in the microtubular network and were carried forward in unison as the hypha elongated. During branching, one or more cortical Mts became associated with the incipient branch and were pulled into the emergence of the branch. As extension of the branch and distortion of the Mts continued, Mts soon were severed with both new Mt ends (+ and -) present in the new branch. Although the exact mechanisms for addition Mt recruitment into the branch remains an open question, the recorded evidence indicates both bulk insertion of established cortical parent-hypha Mts as well as in situ polymerization were involved. The latter conclusion was supported by FRAP studies showing evidence of Mt nucleation and polymerization assembly in the growing tip of the developing branch. Nuclei entered the branch entrapped in the advancing network of Mts.

Original languageEnglish (US)
Pages (from-to)389-400
Number of pages12
JournalFungal Genetics and Biology
Volume43
Issue number6
DOIs
StatePublished - Jun 2006

Fingerprint

Neurospora crassa
Microtubules
Hyphae
Growth
Cytoskeleton
Polymerization
Confocal Microscopy
Cytoplasm

Keywords

  • Branching
  • GFP-tubulin
  • Microtubule dynamics
  • Neurospora crassa

ASJC Scopus subject areas

  • Genetics
  • Microbiology

Cite this

Microtubule dynamics and organization during hyphal growth and branching in Neurospora crassa. / Mouriño-Pérez, Rosa R.; Roberson, Robert; Bartnicki-García, Salomon.

In: Fungal Genetics and Biology, Vol. 43, No. 6, 06.2006, p. 389-400.

Research output: Contribution to journalArticle

Mouriño-Pérez, Rosa R. ; Roberson, Robert ; Bartnicki-García, Salomon. / Microtubule dynamics and organization during hyphal growth and branching in Neurospora crassa. In: Fungal Genetics and Biology. 2006 ; Vol. 43, No. 6. pp. 389-400.
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