Microtubule Disassembly: A Quantitative Kinetic Approach for Defining Endwise Linear Depolymerization

Daniel L. Purich, Timothy L. Karr, David Kristofferson

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

This chapter presents the pertinent theoretical aspects and describes the experimental approaches to characterize the mechanism of depolymerization. To analyze endwise depolymerization quantitatively, various premises must hold: (1) the polymer undergoes stepwise disassembly in a series first-order fashion, (2) the off-rate constant is independent of polymer length over the course of depolymerization, (3) the on-rate constant is zero, (4) turbidity is a measure of the remaining polymer weight concentration and is independent of the polymer length distribution, and (5) the concentrations of the various polymer lengths may be estimated by use of electron microscopy. Primarily, the chapter examines the kinetics of the series first-order decay of polymer and its expression in terms of remaining polymer weight concentration. For indefinite polymerization processes that result from entropydriven condensation equilibria, there are three ways to effect depolymerization: (1) dilution to below the critical concentration, (2) reduction of the temperature to destabilize the polymer, and (3) addition of a reagent to reduce the concentration of the form of the protomer in equilibrium with the polymer. The methods for bringing about such changes must be rapid relative to the time course of depolymerization; otherwise, the kinetics of effecting the depolymerization process will obscure the kinetics of polymer loss.

Original languageEnglish (US)
Pages (from-to)439-450
Number of pages12
JournalMethods in Enzymology
Volume85
Issue numberC
DOIs
StatePublished - Jan 1 1982
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Fingerprint Dive into the research topics of 'Microtubule Disassembly: A Quantitative Kinetic Approach for Defining Endwise Linear Depolymerization'. Together they form a unique fingerprint.

  • Cite this