TY - JOUR
T1 - Microtubule depolymerization in Uromyces appendiculatus by three new antineoplastic drugs
T2 - Combretastatin A-4, dolastatin 10 and halichondrin B
AU - Roberson, Robert
AU - Tucker, Bruce
AU - Pettit, George
PY - 1998/3
Y1 - 1998/3
N2 - Three new antineoplastic natural products, combretastatin A-4, dolastatin 10 and halichondrin B, have been evaluated for their antimicrotubule activity in Uromyces appendiculatus urediniospore germlings using indirect immunofluorescence microscopy. In control germlings microtubules were abundant and mostly oriented parallel to the longitudinal axis of the cell. The microtubule cytoskeleton of germlings treated with 1.3 x 10-5 M (10 μg ml-1) dolastatin 10 and 4.5 x 10-5 M (50 μg ml-1) halichondrin B disrupted the microtubule cytoskeleton resulting in the near elimination of microtubule-associated fluorescence. Combretastatin A-4 was less effective, requiring a concentration of 3.2 x 10-3 M (1.0 mg ml-1) to disrupt the microtubule cytoskeleton. These effective doses are consistent with previously examined antimicrotubule agents (e.g. nocodazole, griseofulvin, vincristine sulphate, demecolcine).
AB - Three new antineoplastic natural products, combretastatin A-4, dolastatin 10 and halichondrin B, have been evaluated for their antimicrotubule activity in Uromyces appendiculatus urediniospore germlings using indirect immunofluorescence microscopy. In control germlings microtubules were abundant and mostly oriented parallel to the longitudinal axis of the cell. The microtubule cytoskeleton of germlings treated with 1.3 x 10-5 M (10 μg ml-1) dolastatin 10 and 4.5 x 10-5 M (50 μg ml-1) halichondrin B disrupted the microtubule cytoskeleton resulting in the near elimination of microtubule-associated fluorescence. Combretastatin A-4 was less effective, requiring a concentration of 3.2 x 10-3 M (1.0 mg ml-1) to disrupt the microtubule cytoskeleton. These effective doses are consistent with previously examined antimicrotubule agents (e.g. nocodazole, griseofulvin, vincristine sulphate, demecolcine).
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U2 - 10.1017/S0953756297004930
DO - 10.1017/S0953756297004930
M3 - Article
AN - SCOPUS:0031791040
VL - 102
SP - 378
EP - 382
JO - Fungal Biology
JF - Fungal Biology
SN - 1878-6146
IS - 3
ER -