TY - JOUR
T1 - Microtubule depolymerization in Uromyces appendiculatus by three new antineoplastic drugs
T2 - Combretastatin A-4, dolastatin 10 and halichondrin B
AU - Roberson, Robert
AU - Tucker, Bruce
AU - Pettit, George
N1 - Funding Information:
We thank Dr John P. Shields (Department of Plant Pathology, University of Georgia, Athens, GA 30602) for critically reading this manuscript and providing useful comments. This work was supported by grants from Arizona State University (FGIA HNR-E042 ; R. W. R.) ; an Outstanding Investigator Grant (CA44344-01A1-08) awarded by the U.S. National Cancer Institute, Division of Cancer Treatment, Diagnosis and Centers, DHHS (G. R. P.) ; the Arizona Disease Control Research Commission (G. R. P.) ; and the Robert B. Dalton Endowment Fund (G. R. P.).
PY - 1998/3
Y1 - 1998/3
N2 - Three new antineoplastic natural products, combretastatin A-4, dolastatin 10 and halichondrin B, have been evaluated for their antimicrotubule activity in Uromyces appendiculatus urediniospore germlings using indirect immunofluorescence microscopy. In control germlings microtubules were abundant and mostly oriented parallel to the longitudinal axis of the cell. The microtubule cytoskeleton of germlings treated with 1.3 x 10-5 M (10 μg ml-1) dolastatin 10 and 4.5 x 10-5 M (50 μg ml-1) halichondrin B disrupted the microtubule cytoskeleton resulting in the near elimination of microtubule-associated fluorescence. Combretastatin A-4 was less effective, requiring a concentration of 3.2 x 10-3 M (1.0 mg ml-1) to disrupt the microtubule cytoskeleton. These effective doses are consistent with previously examined antimicrotubule agents (e.g. nocodazole, griseofulvin, vincristine sulphate, demecolcine).
AB - Three new antineoplastic natural products, combretastatin A-4, dolastatin 10 and halichondrin B, have been evaluated for their antimicrotubule activity in Uromyces appendiculatus urediniospore germlings using indirect immunofluorescence microscopy. In control germlings microtubules were abundant and mostly oriented parallel to the longitudinal axis of the cell. The microtubule cytoskeleton of germlings treated with 1.3 x 10-5 M (10 μg ml-1) dolastatin 10 and 4.5 x 10-5 M (50 μg ml-1) halichondrin B disrupted the microtubule cytoskeleton resulting in the near elimination of microtubule-associated fluorescence. Combretastatin A-4 was less effective, requiring a concentration of 3.2 x 10-3 M (1.0 mg ml-1) to disrupt the microtubule cytoskeleton. These effective doses are consistent with previously examined antimicrotubule agents (e.g. nocodazole, griseofulvin, vincristine sulphate, demecolcine).
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U2 - 10.1017/S0953756297004930
DO - 10.1017/S0953756297004930
M3 - Article
AN - SCOPUS:0031791040
SN - 0953-7562
VL - 102
SP - 378
EP - 382
JO - Mycological Research
JF - Mycological Research
IS - 3
ER -