Microsecond resolution of single-molecule rotation catalyzed by molecular motors

Tassilo Hornung, James Martin, David Spetzler, Robert Ishmukhametov, Wayne Frasch

Research output: Chapter in Book/Report/Conference proceedingChapter

10 Citations (Scopus)

Abstract

Single-molecule measurements of rotation catalyzed by the F 1-ATPase or the F oF 1 ATP synthase have provided new insights into the molecular mechanisms of the F 1 and F o molecular motors. We recently developed a method to record ATPase-driven rotation of F 1 or F oF 1 in a manner that solves several technical limitations of earlier approaches that were significantly hampered by time and angular resolution, and restricted the duration of data collection. With our approach it is possible to collect data for hours and obtain statistically significant quantities of data on each molecule examined with a time resolution of up to 5 μs at unprecedented signal-to-noise.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
Pages273-289
Number of pages17
Volume778
DOIs
StatePublished - 2011

Publication series

NameMethods in Molecular Biology
Volume778
ISSN (Print)10643745

Fingerprint

Proton-Translocating ATPases
Adenosine Triphosphatases
Noise
Adenosine Triphosphate

Keywords

  • Dark field microscopy
  • F -ATPase
  • F F ATP synthase
  • Gold nanorods
  • Molecular motors
  • Nanodiscs
  • Plasmon resonance
  • Single molecule

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Hornung, T., Martin, J., Spetzler, D., Ishmukhametov, R., & Frasch, W. (2011). Microsecond resolution of single-molecule rotation catalyzed by molecular motors. In Methods in Molecular Biology (Vol. 778, pp. 273-289). (Methods in Molecular Biology; Vol. 778). https://doi.org/10.1007/978-1-61779-261-8_18

Microsecond resolution of single-molecule rotation catalyzed by molecular motors. / Hornung, Tassilo; Martin, James; Spetzler, David; Ishmukhametov, Robert; Frasch, Wayne.

Methods in Molecular Biology. Vol. 778 2011. p. 273-289 (Methods in Molecular Biology; Vol. 778).

Research output: Chapter in Book/Report/Conference proceedingChapter

Hornung, T, Martin, J, Spetzler, D, Ishmukhametov, R & Frasch, W 2011, Microsecond resolution of single-molecule rotation catalyzed by molecular motors. in Methods in Molecular Biology. vol. 778, Methods in Molecular Biology, vol. 778, pp. 273-289. https://doi.org/10.1007/978-1-61779-261-8_18
Hornung T, Martin J, Spetzler D, Ishmukhametov R, Frasch W. Microsecond resolution of single-molecule rotation catalyzed by molecular motors. In Methods in Molecular Biology. Vol. 778. 2011. p. 273-289. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-61779-261-8_18
Hornung, Tassilo ; Martin, James ; Spetzler, David ; Ishmukhametov, Robert ; Frasch, Wayne. / Microsecond resolution of single-molecule rotation catalyzed by molecular motors. Methods in Molecular Biology. Vol. 778 2011. pp. 273-289 (Methods in Molecular Biology).
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