Microscopic linear liquid streams in vacuum: Injection of solvated biological samples into X-ray free electron lasers

R. B. Doak, D. P. Deponte, G. Nelson, F. Camacho-Alanis, Alexandra Ros, John Spence, Uwe Weierstall

Research output: Chapter in Book/Report/Conference proceedingConference contribution

7 Citations (Scopus)

Abstract

Microscopic linear liquid free-streams offer a means of gently delivering biological samples into a probe beam in vacuum while maintaining the sample species in a fully solvated state. By employing gas dynamic forces to form the microscopic liquid stream (as opposed to a conventional solid-walled convergent nozzle), liquid free-streams down to 300 nm diameter have been generated. Such "Gas Dynamic Virtual Nozzles" (GDVN) are ideally suited to injecting complex biological species into an X-ray Free Electron Laser (XFEL) to determine the structure of the biological species via Serial Femtosecond Crystallography (SFX). GDVN injector technology developed for this purpose is described.

Original languageEnglish (US)
Title of host publicationAIP Conference Proceedings
Pages1314-1323
Number of pages10
Volume1501
Edition1
DOIs
StatePublished - 2012
Event28th International Symposium on Rarefied Gas Dynamics 2012, RGD 2012 - Zaragoza, Spain
Duration: Jul 9 2012Jul 13 2012

Other

Other28th International Symposium on Rarefied Gas Dynamics 2012, RGD 2012
CountrySpain
CityZaragoza
Period7/9/127/13/12

Fingerprint

gas dynamics
free electron lasers
free flow
injection
vacuum
nozzles
convergent nozzles
liquids
x rays
injectors
crystallography
probes

Keywords

  • Biological Structure Determination
  • Gas-Dynamic Virtual Nozzle
  • Nanocrystallography
  • Rayleigh Droplet Streams
  • Rayleigh-Plateau Instability
  • Serial Femtosecond Crystallography
  • X-ray Free Electron Laser

ASJC Scopus subject areas

  • Physics and Astronomy(all)

Cite this

Doak, R. B., Deponte, D. P., Nelson, G., Camacho-Alanis, F., Ros, A., Spence, J., & Weierstall, U. (2012). Microscopic linear liquid streams in vacuum: Injection of solvated biological samples into X-ray free electron lasers. In AIP Conference Proceedings (1 ed., Vol. 1501, pp. 1314-1323) https://doi.org/10.1063/1.4769693

Microscopic linear liquid streams in vacuum : Injection of solvated biological samples into X-ray free electron lasers. / Doak, R. B.; Deponte, D. P.; Nelson, G.; Camacho-Alanis, F.; Ros, Alexandra; Spence, John; Weierstall, Uwe.

AIP Conference Proceedings. Vol. 1501 1. ed. 2012. p. 1314-1323.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Doak, RB, Deponte, DP, Nelson, G, Camacho-Alanis, F, Ros, A, Spence, J & Weierstall, U 2012, Microscopic linear liquid streams in vacuum: Injection of solvated biological samples into X-ray free electron lasers. in AIP Conference Proceedings. 1 edn, vol. 1501, pp. 1314-1323, 28th International Symposium on Rarefied Gas Dynamics 2012, RGD 2012, Zaragoza, Spain, 7/9/12. https://doi.org/10.1063/1.4769693
Doak RB, Deponte DP, Nelson G, Camacho-Alanis F, Ros A, Spence J et al. Microscopic linear liquid streams in vacuum: Injection of solvated biological samples into X-ray free electron lasers. In AIP Conference Proceedings. 1 ed. Vol. 1501. 2012. p. 1314-1323 https://doi.org/10.1063/1.4769693
Doak, R. B. ; Deponte, D. P. ; Nelson, G. ; Camacho-Alanis, F. ; Ros, Alexandra ; Spence, John ; Weierstall, Uwe. / Microscopic linear liquid streams in vacuum : Injection of solvated biological samples into X-ray free electron lasers. AIP Conference Proceedings. Vol. 1501 1. ed. 2012. pp. 1314-1323
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