Microscopic and spectroscopic studies of untreated and hexanol-treated chlorosomes from Chloroflexus aurantiacus

Yinwen Zhu, B. L. Ramakrishna, Paula I. van Noort, Robert E. Blankenship

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

When isolated chlorosomes from Chloroflexus aurantiacus are treated with 1-hexanol, the BChl cQy absorption band shifts from 740 to 670 nm, while the baseplate BChl a remains at 795 nm. The relative amount of BChl c in the 740 and 670 nm forms depends on the hexanol concentration. Atomic force microscopy was used to study the ultrastructure of native, hexanol-treated, and protein-free chlorosomes. Chlorosomes appeared to be larger and more rounded upon hexanol treatment and did not return to the original shape or size after 2-fold dilution. Therefore, the hexanol treatment is not completely reversible in terms of chlorosome structure. Untreated, hexanol-treated and and hexanol-treated and then diluted samples were investigated using steady-state and time-resolved fluorescence spectroscopy. For the sample treated with 68 mM hexanol, a 24 ps energy transfer from BChl c to a was observed in the picosecond fluorescence measurements. After 2-fold dilution, most of the kinetic properties of the untreated chlorosomes, characterized by a major energy transfer component of 15 ps from BChl c 740 to BChl a 795, were regained. Energy transfer from either BChI c 740 or BChl c 670 to baseplate BChl a is fast and relatively efficient in untreated chlorosomes. In hexanol-treated chlorosomes, the excited state lifetime is not very different from that in untreated samples, but the energy transfer efficiency is quite low. This may result from concentration quenching of the monomeric pigments in the hexanol-treated Chorosomes.

Original languageEnglish (US)
Pages (from-to)197-207
Number of pages11
JournalBBA - Bioenergetics
Volume1232
Issue number3
DOIs
StatePublished - Dec 12 1995

Fingerprint

Hexanols
Energy Transfer
Energy transfer
Dilution
Chloroflexus
Atomic Force Microscopy
Fluorescence Spectrometry
Fluorescence spectroscopy
Excited states
Pigments
Absorption spectra
Quenching
Atomic force microscopy
Fluorescence

Keywords

  • Atomic force microscopy
  • Bacteriochlorophyll
  • Chlorosome
  • Energy transfer
  • Hexanol
  • Photosynthesis
  • Ultrastructure

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology

Cite this

Microscopic and spectroscopic studies of untreated and hexanol-treated chlorosomes from Chloroflexus aurantiacus. / Zhu, Yinwen; Ramakrishna, B. L.; van Noort, Paula I.; Blankenship, Robert E.

In: BBA - Bioenergetics, Vol. 1232, No. 3, 12.12.1995, p. 197-207.

Research output: Contribution to journalArticle

Zhu, Yinwen ; Ramakrishna, B. L. ; van Noort, Paula I. ; Blankenship, Robert E. / Microscopic and spectroscopic studies of untreated and hexanol-treated chlorosomes from Chloroflexus aurantiacus. In: BBA - Bioenergetics. 1995 ; Vol. 1232, No. 3. pp. 197-207.
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