Microscopic Analysis of Viral Infection

Honor L. Glenn, Ana Lemos de Matos, Nancy Villa, Grant McFadden

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Viruses engineered to express fluorescent proteins can be used with live-cell imaging techniques to monitor the progression of infection in real time. Here we describe a set of methods to track infection spreading from one cell population to another as well as to visualize transfer of virions between cells. This approach is extended to multiplexing with physiological readouts of cell death, which can be correlated with single-cell resolution to viral infection.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages163-177
Number of pages15
DOIs
StatePublished - 2021

Publication series

NameMethods in Molecular Biology
Volume2225
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Apoptosis
  • Cell death
  • Confocal
  • Fluorescence
  • Infection
  • Live-cell imaging
  • Myxoma virus
  • Viruses

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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