Microscale Oil-Covered Cell Array (MOCCA): A droplet array for high-content single-cell analysis and imaging

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A simple, low-cost technique for high throughput single-cell analysis, Microscale Oil-Covered Cell Array (MOCCA), is presented in this paper. Corresponding to recent research on single cell analysis, simple devices for isolated cell chambers are urgently needed and long sought-after. Instead of using microfabricated solid structures to capture cells, MOCCA isolates cells in discrete aqueous droplets that are separated by oil on the patterned hydrophilic areas on a relatively more hydrophobic flat substrate. In our pioneer study, we created an array of 700-picoliter droplets. The randomly seeded E. coli cell number in each discrete droplet approaches single-cell levels. The total time needed for MOCCA fabrication was no more than 10 minutes. Compared to traditional micro-fabrication techniques, MOCCA dramatically lowers the cost and enhances the efficiency for the fabrication procedure, while producing a microscale array as in those made using traditional methods.

Original languageEnglish (US)
Title of host publicationProceedings of the 7th International Conference on Nanochannels, Microchannels, and Minichannels 2009, ICNMM2009
Pages669-672
Number of pages4
EditionPART A
DOIs
StatePublished - Dec 1 2009
Event7th International Conference on Nanochannels, Microchannels, and Minichannels, ICNMM2009 - Pohang, Korea, Republic of
Duration: Jun 22 2009Jun 24 2009

Publication series

NameProceedings of the 7th International Conference on Nanochannels, Microchannels, and Minichannels 2009, ICNMM2009
NumberPART A

Other

Other7th International Conference on Nanochannels, Microchannels, and Minichannels, ICNMM2009
CountryKorea, Republic of
CityPohang
Period6/22/096/24/09

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ASJC Scopus subject areas

  • Fluid Flow and Transfer Processes
  • Mechanical Engineering

Cite this

Lin, L. I., Chao, S-H., & Meldrum, D. (2009). Microscale Oil-Covered Cell Array (MOCCA): A droplet array for high-content single-cell analysis and imaging. In Proceedings of the 7th International Conference on Nanochannels, Microchannels, and Minichannels 2009, ICNMM2009 (PART A ed., pp. 669-672). (Proceedings of the 7th International Conference on Nanochannels, Microchannels, and Minichannels 2009, ICNMM2009; No. PART A). https://doi.org/10.1115/ICNMM2009-82283