Microreactor array device

Peter Wiktor; Al Brunner; Peter Kahn; Ji Qiu; Dewey Magee; Xiaofang Bian; Kailash Karthikeyan; Joshua LaBaer

doi:10.1038/srep08736

Microreactor array device

Peter Wiktor, Al Brunner, Peter Kahn, Ji Qiu, Dewey Magee, Xiaofang Bian, Kailash Karthikeyan, Joshua LaBaer

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

We report a device to fill an array of small chemical reaction chambers (microreactors) with reagent and then seal them using pressurized viscous liquid acting through a flexible membrane. The device enables multiple, independent chemical reactions involving free floating intermediate molecules without interference from neighboring reactions or external environments. The device is validated by protein expressed in situ directly from DNA in a microarray of ∼10,000 spots with no diffusion during three hours incubation. Using the device to probe for an autoantibody cancer biomarker in blood serum sample gave five times higher signal to background ratio compared to standard protein microarray expressed on a flat microscope slide. Physical design principles to effectively fill the array of microreactors with reagent and experimental results of alternate methods for sealing the microreactors are presented.

Original language	English (US)
Article number	8736
Journal	Scientific reports
Volume	5
DOIs	https://doi.org/10.1038/srep08736
State	Published - 2015

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10.1038/srep08736

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title = "Microreactor array device",

abstract = "We report a device to fill an array of small chemical reaction chambers (microreactors) with reagent and then seal them using pressurized viscous liquid acting through a flexible membrane. The device enables multiple, independent chemical reactions involving free floating intermediate molecules without interference from neighboring reactions or external environments. The device is validated by protein expressed in situ directly from DNA in a microarray of ∼10,000 spots with no diffusion during three hours incubation. Using the device to probe for an autoantibody cancer biomarker in blood serum sample gave five times higher signal to background ratio compared to standard protein microarray expressed on a flat microscope slide. Physical design principles to effectively fill the array of microreactors with reagent and experimental results of alternate methods for sealing the microreactors are presented.",

author = "Peter Wiktor and Al Brunner and Peter Kahn and Ji Qiu and Dewey Magee and Xiaofang Bian and Kailash Karthikeyan and Joshua LaBaer",

note = "Funding Information: This work is partially funded by grant number R42GM106704 awarded by the National Institutes of Health to Engineering Arts LLC. Silicon wafer processing was provided by Center for Solid State Electronics Research (CSSER), Arizona State University (ASU), Tempe AZ. We thank Linda Gregg for her support.",

year = "2015",

doi = "10.1038/srep08736",

language = "English (US)",

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journal = "Scientific reports",

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AU - Wiktor, Peter

AU - Brunner, Al

AU - Kahn, Peter

AU - Qiu, Ji

AU - Magee, Dewey

AU - Bian, Xiaofang

AU - Karthikeyan, Kailash

AU - LaBaer, Joshua

N1 - Funding Information: This work is partially funded by grant number R42GM106704 awarded by the National Institutes of Health to Engineering Arts LLC. Silicon wafer processing was provided by Center for Solid State Electronics Research (CSSER), Arizona State University (ASU), Tempe AZ. We thank Linda Gregg for her support.

PY - 2015

Y1 - 2015

N2 - We report a device to fill an array of small chemical reaction chambers (microreactors) with reagent and then seal them using pressurized viscous liquid acting through a flexible membrane. The device enables multiple, independent chemical reactions involving free floating intermediate molecules without interference from neighboring reactions or external environments. The device is validated by protein expressed in situ directly from DNA in a microarray of ∼10,000 spots with no diffusion during three hours incubation. Using the device to probe for an autoantibody cancer biomarker in blood serum sample gave five times higher signal to background ratio compared to standard protein microarray expressed on a flat microscope slide. Physical design principles to effectively fill the array of microreactors with reagent and experimental results of alternate methods for sealing the microreactors are presented.

AB - We report a device to fill an array of small chemical reaction chambers (microreactors) with reagent and then seal them using pressurized viscous liquid acting through a flexible membrane. The device enables multiple, independent chemical reactions involving free floating intermediate molecules without interference from neighboring reactions or external environments. The device is validated by protein expressed in situ directly from DNA in a microarray of ∼10,000 spots with no diffusion during three hours incubation. Using the device to probe for an autoantibody cancer biomarker in blood serum sample gave five times higher signal to background ratio compared to standard protein microarray expressed on a flat microscope slide. Physical design principles to effectively fill the array of microreactors with reagent and experimental results of alternate methods for sealing the microreactors are presented.

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Microreactor array device

Abstract

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