Abstract
Both Cowania subintegra Kearney and C. stansburiana Torr. were successfully propagated in vitro. Shoot proliferation occurred from shoot tips of green-house grown C. subintegra using a modified Murashige and Skoog medium supplemented with 4.4 μM 6-benzyladenine and 0.5 μM indole butyric acid. Excised microshoots (1.5-3.0 cm long) of both species were rooted using a two-step process in which they were cultured for 3 days in a root initiation medium with 2.7 μM naphthaleneacetic acid and then transferred to a low nitrogen root elongation medium without auxin. Plantlets were successfully transferred to soilless potting mix.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 297-299 |
| Number of pages | 3 |
| Journal | Plant Cell, Tissue and Organ Culture |
| Volume | 35 |
| Issue number | 3 |
| DOIs | |
| State | Published - Dec 1993 |
Fingerprint
Keywords
- actinorhizae
- cliffrose
- Rosaceae
- tissue culture
ASJC Scopus subject areas
- Biotechnology
- Plant Science
Cite this
Micropropagation of Cowania subintegra and Cowania stansburiana. / Jakobek, Judy L.; Stutz, Jean C.; Bess, Vicki H.; Backhaus, Ralph A.
In: Plant Cell, Tissue and Organ Culture, Vol. 35, No. 3, 12.1993, p. 297-299.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Micropropagation of Cowania subintegra and Cowania stansburiana
AU - Jakobek, Judy L.
AU - Stutz, Jean C.
AU - Bess, Vicki H.
AU - Backhaus, Ralph A.
PY - 1993/12
Y1 - 1993/12
N2 - Both Cowania subintegra Kearney and C. stansburiana Torr. were successfully propagated in vitro. Shoot proliferation occurred from shoot tips of green-house grown C. subintegra using a modified Murashige and Skoog medium supplemented with 4.4 μM 6-benzyladenine and 0.5 μM indole butyric acid. Excised microshoots (1.5-3.0 cm long) of both species were rooted using a two-step process in which they were cultured for 3 days in a root initiation medium with 2.7 μM naphthaleneacetic acid and then transferred to a low nitrogen root elongation medium without auxin. Plantlets were successfully transferred to soilless potting mix.
AB - Both Cowania subintegra Kearney and C. stansburiana Torr. were successfully propagated in vitro. Shoot proliferation occurred from shoot tips of green-house grown C. subintegra using a modified Murashige and Skoog medium supplemented with 4.4 μM 6-benzyladenine and 0.5 μM indole butyric acid. Excised microshoots (1.5-3.0 cm long) of both species were rooted using a two-step process in which they were cultured for 3 days in a root initiation medium with 2.7 μM naphthaleneacetic acid and then transferred to a low nitrogen root elongation medium without auxin. Plantlets were successfully transferred to soilless potting mix.
KW - actinorhizae
KW - cliffrose
KW - Rosaceae
KW - tissue culture
UR - http://www.scopus.com/inward/record.url?scp=0027140078&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027140078&partnerID=8YFLogxK
U2 - 10.1007/BF00037285
DO - 10.1007/BF00037285
M3 - Article
AN - SCOPUS:0027140078
VL - 35
SP - 297
EP - 299
JO - Plant Cell, Tissue and Organ Culture
JF - Plant Cell, Tissue and Organ Culture
SN - 0167-6857
IS - 3
ER -