Micropropagation of Cowania subintegra and Cowania stansburiana

Judy L. Jakobek; Jean C. Stutz; Vicki H. Bess; Ralph A. Backhaus

doi:10.1007/BF00037285

Micropropagation of Cowania subintegra and Cowania stansburiana

Judy L. Jakobek, Jean C. Stutz, Vicki H. Bess, Ralph A. Backhaus

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Both Cowania subintegra Kearney and C. stansburiana Torr. were successfully propagated in vitro. Shoot proliferation occurred from shoot tips of green-house grown C. subintegra using a modified Murashige and Skoog medium supplemented with 4.4 μM 6-benzyladenine and 0.5 μM indole butyric acid. Excised microshoots (1.5-3.0 cm long) of both species were rooted using a two-step process in which they were cultured for 3 days in a root initiation medium with 2.7 μM naphthaleneacetic acid and then transferred to a low nitrogen root elongation medium without auxin. Plantlets were successfully transferred to soilless potting mix.

Original language	English (US)
Pages (from-to)	297-299
Number of pages	3
Journal	Plant Cell, Tissue and Organ Culture
Volume	35
Issue number	3
DOIs	https://doi.org/10.1007/BF00037285
State	Published - Dec 1 1993

Keywords

Rosaceae
actinorhizae
cliffrose
tissue culture

ASJC Scopus subject areas

Horticulture

Access to Document

10.1007/BF00037285

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title = "Micropropagation of Cowania subintegra and Cowania stansburiana",

abstract = "Both Cowania subintegra Kearney and C. stansburiana Torr. were successfully propagated in vitro. Shoot proliferation occurred from shoot tips of green-house grown C. subintegra using a modified Murashige and Skoog medium supplemented with 4.4 μM 6-benzyladenine and 0.5 μM indole butyric acid. Excised microshoots (1.5-3.0 cm long) of both species were rooted using a two-step process in which they were cultured for 3 days in a root initiation medium with 2.7 μM naphthaleneacetic acid and then transferred to a low nitrogen root elongation medium without auxin. Plantlets were successfully transferred to soilless potting mix.",

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T1 - Micropropagation of Cowania subintegra and Cowania stansburiana

AU - Jakobek, Judy L.

AU - Stutz, Jean C.

AU - Bess, Vicki H.

AU - Backhaus, Ralph A.

PY - 1993/12/1

Y1 - 1993/12/1

N2 - Both Cowania subintegra Kearney and C. stansburiana Torr. were successfully propagated in vitro. Shoot proliferation occurred from shoot tips of green-house grown C. subintegra using a modified Murashige and Skoog medium supplemented with 4.4 μM 6-benzyladenine and 0.5 μM indole butyric acid. Excised microshoots (1.5-3.0 cm long) of both species were rooted using a two-step process in which they were cultured for 3 days in a root initiation medium with 2.7 μM naphthaleneacetic acid and then transferred to a low nitrogen root elongation medium without auxin. Plantlets were successfully transferred to soilless potting mix.

AB - Both Cowania subintegra Kearney and C. stansburiana Torr. were successfully propagated in vitro. Shoot proliferation occurred from shoot tips of green-house grown C. subintegra using a modified Murashige and Skoog medium supplemented with 4.4 μM 6-benzyladenine and 0.5 μM indole butyric acid. Excised microshoots (1.5-3.0 cm long) of both species were rooted using a two-step process in which they were cultured for 3 days in a root initiation medium with 2.7 μM naphthaleneacetic acid and then transferred to a low nitrogen root elongation medium without auxin. Plantlets were successfully transferred to soilless potting mix.

KW - Rosaceae

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