Microprinting of on-chip cultures: Patterning of yeast cell microarrays using concanavalin-a adhesion

Tracy H. Fung, Gregory I. Ball, Sarah C. McQuaide, Shih-Hui Chao, Alejandro Colman-Lerner, Mark R. Holl, Deirdre Meldrum

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

Microprinting has been demonstrated effective in the patterning of surface regions for trapping cells used within microfluidic devices. In this study a polydimethylsiloxane (PDMS) silicone elastomer stamp was microfabricated and used to microstamp concanavalin-A (con-A; protein that binds to yeast) on a glass surface. Yeast cells, Saccharomyces cerevisiae, were brought into contact with patterned con-A producing an array of yeast microscale culture in an ordered array identical to the printed pattern.

Original languageEnglish (US)
Title of host publicationAdvances in Bioengineering, BED
PublisherAmerican Society of Mechanical Engineers (ASME)
Pages373-374
Number of pages2
StatePublished - 2004
Externally publishedYes
Event2004 ASME International Mechanical Engineering Congress and Exposition, IMECE - Anaheim, CA, United States
Duration: Nov 13 2004Nov 19 2004

Other

Other2004 ASME International Mechanical Engineering Congress and Exposition, IMECE
CountryUnited States
CityAnaheim, CA
Period11/13/0411/19/04

ASJC Scopus subject areas

  • Engineering(all)

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