Microprinting of on-chip cultures

Patterning of yeast cell microarrays using concanavalin-a adhesion

Tracy H. Fung, Gregory I. Ball, Sarah C. McQuaide, Shih-Hui Chao, Alejandro Colman-Lerner, Mark R. Holl, Deirdre Meldrum

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Microprinting has been demonstrated effective in the patterning of surface regions for trapping cells used within microfluidic devices. In this study a polydimethylsiloxane (PDMS) silicone elastomer stamp was microfabricated and used to microstamp concanavalin-A (con-A; protein that binds to yeast) on a glass surface. Yeast cells, Saccharomyces cerevisiae, were brought into contact with patterned con-A producing an array of yeast microscale culture in an ordered array identical to the printed pattern.

Original languageEnglish (US)
Title of host publicationAdvances in Bioengineering, BED
PublisherAmerican Society of Mechanical Engineers (ASME)
Pages373-374
Number of pages2
StatePublished - 2004
Externally publishedYes
Event2004 ASME International Mechanical Engineering Congress and Exposition, IMECE - Anaheim, CA, United States
Duration: Nov 13 2004Nov 19 2004

Other

Other2004 ASME International Mechanical Engineering Congress and Exposition, IMECE
CountryUnited States
CityAnaheim, CA
Period11/13/0411/19/04

Fingerprint

Microarrays
Cell culture
Yeast
Adhesion
Cells
Polydimethylsiloxane
Microfluidics
Elastomers
Silicones
Proteins
Glass

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Fung, T. H., Ball, G. I., McQuaide, S. C., Chao, S-H., Colman-Lerner, A., Holl, M. R., & Meldrum, D. (2004). Microprinting of on-chip cultures: Patterning of yeast cell microarrays using concanavalin-a adhesion. In Advances in Bioengineering, BED (pp. 373-374). [IMECE2004-60866] American Society of Mechanical Engineers (ASME).

Microprinting of on-chip cultures : Patterning of yeast cell microarrays using concanavalin-a adhesion. / Fung, Tracy H.; Ball, Gregory I.; McQuaide, Sarah C.; Chao, Shih-Hui; Colman-Lerner, Alejandro; Holl, Mark R.; Meldrum, Deirdre.

Advances in Bioengineering, BED. American Society of Mechanical Engineers (ASME), 2004. p. 373-374 IMECE2004-60866.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Fung, TH, Ball, GI, McQuaide, SC, Chao, S-H, Colman-Lerner, A, Holl, MR & Meldrum, D 2004, Microprinting of on-chip cultures: Patterning of yeast cell microarrays using concanavalin-a adhesion. in Advances in Bioengineering, BED., IMECE2004-60866, American Society of Mechanical Engineers (ASME), pp. 373-374, 2004 ASME International Mechanical Engineering Congress and Exposition, IMECE, Anaheim, CA, United States, 11/13/04.
Fung TH, Ball GI, McQuaide SC, Chao S-H, Colman-Lerner A, Holl MR et al. Microprinting of on-chip cultures: Patterning of yeast cell microarrays using concanavalin-a adhesion. In Advances in Bioengineering, BED. American Society of Mechanical Engineers (ASME). 2004. p. 373-374. IMECE2004-60866
Fung, Tracy H. ; Ball, Gregory I. ; McQuaide, Sarah C. ; Chao, Shih-Hui ; Colman-Lerner, Alejandro ; Holl, Mark R. ; Meldrum, Deirdre. / Microprinting of on-chip cultures : Patterning of yeast cell microarrays using concanavalin-a adhesion. Advances in Bioengineering, BED. American Society of Mechanical Engineers (ASME), 2004. pp. 373-374
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