Microprinting of on-chip cultures: Patterning of yeast cell microarrays using concanavalin-a adhesion

Tracy H. Fung; Gregory I. Ball; Sarah C. McQuaide; Shih-Hui Chao; Alejandro Colman-Lerner; Mark R. Holl; Deirdre Meldrum

Microprinting of on-chip cultures: Patterning of yeast cell microarrays using concanavalin-a adhesion

Tracy H. Fung, Gregory I. Ball, Sarah C. McQuaide, Shih-Hui Chao, Alejandro Colman-Lerner, Mark R. Holl, Deirdre Meldrum

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

1 Scopus citations

Abstract

Microprinting has been demonstrated effective in the patterning of surface regions for trapping cells used within microfluidic devices. In this study a polydimethylsiloxane (PDMS) silicone elastomer stamp was microfabricated and used to microstamp concanavalin-A (con-A; protein that binds to yeast) on a glass surface. Yeast cells, Saccharomyces cerevisiae, were brought into contact with patterned con-A producing an array of yeast microscale culture in an ordered array identical to the printed pattern.

Original language	English (US)
Title of host publication	Advances in Bioengineering, BED
Publisher	American Society of Mechanical Engineers (ASME)
Pages	373-374
Number of pages	2
State	Published - 2004
Externally published	Yes
Event	2004 ASME International Mechanical Engineering Congress and Exposition, IMECE - Anaheim, CA, United States Duration: Nov 13 2004 → Nov 19 2004

Other

Other	2004 ASME International Mechanical Engineering Congress and Exposition, IMECE
Country/Territory	United States
City	Anaheim, CA
Period	11/13/04 → 11/19/04

ASJC Scopus subject areas

General Engineering

Cite this

Fung, TH, Ball, GI, McQuaide, SC, Chao, S-H, Colman-Lerner, A, Holl, MR & Meldrum, D 2004, Microprinting of on-chip cultures: Patterning of yeast cell microarrays using concanavalin-a adhesion. in Advances in Bioengineering, BED., IMECE2004-60866, American Society of Mechanical Engineers (ASME), pp. 373-374, 2004 ASME International Mechanical Engineering Congress and Exposition, IMECE, Anaheim, CA, United States, 11/13/04.

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abstract = "Microprinting has been demonstrated effective in the patterning of surface regions for trapping cells used within microfluidic devices. In this study a polydimethylsiloxane (PDMS) silicone elastomer stamp was microfabricated and used to microstamp concanavalin-A (con-A; protein that binds to yeast) on a glass surface. Yeast cells, Saccharomyces cerevisiae, were brought into contact with patterned con-A producing an array of yeast microscale culture in an ordered array identical to the printed pattern.",

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language = "English (US)",

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AU - Chao, Shih-Hui

AU - Colman-Lerner, Alejandro

AU - Holl, Mark R.

AU - Meldrum, Deirdre

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AB - Microprinting has been demonstrated effective in the patterning of surface regions for trapping cells used within microfluidic devices. In this study a polydimethylsiloxane (PDMS) silicone elastomer stamp was microfabricated and used to microstamp concanavalin-A (con-A; protein that binds to yeast) on a glass surface. Yeast cells, Saccharomyces cerevisiae, were brought into contact with patterned con-A producing an array of yeast microscale culture in an ordered array identical to the printed pattern.

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PB - American Society of Mechanical Engineers (ASME)

Y2 - 13 November 2004 through 19 November 2004

ER -

Microprinting of on-chip cultures: Patterning of yeast cell microarrays using concanavalin-a adhesion

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ASJC Scopus subject areas

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