Microglial Beclin 1 Regulates Retromer Trafficking and Phagocytosis and Is Impaired in Alzheimer's Disease

Kurt M. Lucin, Caitlin E. O'Brien, Gregor Bieri, Eva Czirr, Kira I. Mosher, Rachelle J. Abbey, Diego Mastroeni, Joseph Rogers, Brian Spencer, Eliezer Masliah, Tony Wyss-Coray

Research output: Contribution to journalArticle

150 Citations (Scopus)

Abstract

Phagocytosis controls CNS homeostasis by facilitating the removal of unwanted cellular debris. Accordingly, impairments in different receptors or proteins involved in phagocytosis result in enhanced inflammation and neurodegeneration. While various studies have identified extrinsic factors that modulate phagocytosis in health and disease, key intracellular regulators are less understood. Here we show that the autophagy protein beclin 1 is required for efficient phagocytosis invitro and in mouse brains. Furthermore, we show that beclin 1-mediated impairments in phagocytosis are associated with dysfunctional recruitment of retromer to phagosomal membranes, reduced retromer levels, and impaired recycling of phagocytic receptors CD36 and Trem2. Interestingly, microglia isolated from human Alzheimer@s disease (AD) brains show significantly reduced beclin 1 and retromer protein levels. These findings position beclin 1 as a link between autophagy, retromer trafficking, and receptor-mediated phagocytosis and provide insight into mechanisms by which phagocytosis is regulated and how it may become impaired in AD

Original languageEnglish (US)
Pages (from-to)873-886
Number of pages14
JournalNeuron
Volume79
Issue number5
DOIs
StatePublished - Sep 4 2013
Externally publishedYes

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Phagocytosis
Alzheimer Disease
Autophagy
Brain
Microglia
Beclin-1
Homeostasis
Inflammation
Membranes
Health
Proteins

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Lucin, K. M., O'Brien, C. E., Bieri, G., Czirr, E., Mosher, K. I., Abbey, R. J., ... Wyss-Coray, T. (2013). Microglial Beclin 1 Regulates Retromer Trafficking and Phagocytosis and Is Impaired in Alzheimer's Disease. Neuron, 79(5), 873-886. https://doi.org/10.1016/j.neuron.2013.06.046

Microglial Beclin 1 Regulates Retromer Trafficking and Phagocytosis and Is Impaired in Alzheimer's Disease. / Lucin, Kurt M.; O'Brien, Caitlin E.; Bieri, Gregor; Czirr, Eva; Mosher, Kira I.; Abbey, Rachelle J.; Mastroeni, Diego; Rogers, Joseph; Spencer, Brian; Masliah, Eliezer; Wyss-Coray, Tony.

In: Neuron, Vol. 79, No. 5, 04.09.2013, p. 873-886.

Research output: Contribution to journalArticle

Lucin, KM, O'Brien, CE, Bieri, G, Czirr, E, Mosher, KI, Abbey, RJ, Mastroeni, D, Rogers, J, Spencer, B, Masliah, E & Wyss-Coray, T 2013, 'Microglial Beclin 1 Regulates Retromer Trafficking and Phagocytosis and Is Impaired in Alzheimer's Disease', Neuron, vol. 79, no. 5, pp. 873-886. https://doi.org/10.1016/j.neuron.2013.06.046
Lucin, Kurt M. ; O'Brien, Caitlin E. ; Bieri, Gregor ; Czirr, Eva ; Mosher, Kira I. ; Abbey, Rachelle J. ; Mastroeni, Diego ; Rogers, Joseph ; Spencer, Brian ; Masliah, Eliezer ; Wyss-Coray, Tony. / Microglial Beclin 1 Regulates Retromer Trafficking and Phagocytosis and Is Impaired in Alzheimer's Disease. In: Neuron. 2013 ; Vol. 79, No. 5. pp. 873-886.
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