Microfluidic sample delivery for serial crystallography using XFELs

Austin Echelmeier, Mukul Sonker, Alexandra Ros

Research output: Contribution to journalArticle

Abstract

Serial femtosecond crystallography (SFX) with X-ray free electron lasers (XFELs) is an emerging field for structural biology. One of its major impacts lies in the ability to reveal the structure of complex proteins previously inaccessible with synchrotron-based crystallography techniques and allowing time-resolved studies from femtoseconds to seconds. The nature of this serial technique requires new approaches for crystallization, data analysis, and sample delivery. With continued advancements in microfabrication techniques, various developments have been reported in the past decade for innovative and efficient microfluidic sample delivery for crystallography experiments using XFELs. This article summarizes the recent developments in microfluidic sample delivery with liquid injection and fixed-target approaches, which allow exciting new research with XFELs.

Original languageEnglish (US)
JournalAnalytical and bioanalytical chemistry
DOIs
StatePublished - Jan 1 2019
Externally publishedYes

Fingerprint

X ray lasers
Crystallography
Microfluidics
Free electron lasers
X Ray Crystallography
Lasers
X-Rays
Electrons
Microtechnology
Time and motion study
Synchrotrons
Microfabrication
Crystallization
Injections
Liquids
Research
Proteins
Experiments

Keywords

  • Fixed-target
  • Injector
  • Mixing
  • Nozzle
  • Protein
  • Time-resolved

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

Cite this

Microfluidic sample delivery for serial crystallography using XFELs. / Echelmeier, Austin; Sonker, Mukul; Ros, Alexandra.

In: Analytical and bioanalytical chemistry, 01.01.2019.

Research output: Contribution to journalArticle

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