Microfluidic droplet platform for ultrahigh-throughput single-cell screening of biodiversity

Stanislav S. Terekhov, Ivan V. Smirnov, Anastasiya V. Stepanova, Tatyana V. Bobik, Yuliana A. Mokrushina, Natalia A. Ponomarenko, Alexey A. Belogurov, Maria P. Rubtsova, Olga V. Kartseva, Marina O. Gomzikova, Alexey A. Moskovtsev, Anton S. Bukatin, Michael V. Dubina, Elena S. Kostryukova, Vladislav V. Babenko, Maria T. Vakhitova, Alexander I. Manolov, Maja V. Malakhova, Maria A. Kornienko, Alexander V. TyakhtAnna A. Vanyushkina, Elena N. Ilina, Patrick Masson, Alexander G. Gabibov, Sidney Altman

Research output: Contribution to journalArticle

67 Scopus citations

Abstract

Ultrahigh-throughput screening (uHTS) techniques can identify unique functionality from millions of variants. To mimic the natural selection mechanisms that occur by compartmentalization in vivo, we developed a technique based on single-cell encapsulation in droplets of a monodisperse microfluidic double water-in-oil-in-water emulsion (MDE). Biocompatible MDE enables in-droplet cultivation of different living species. The combination of droplet-generating machinery with FACS followed by next-generation sequencing and liquid chromatography-mass spectrometry analysis of the secretomes of encapsulated organisms yielded detailed genotype/phenotype descriptions. This platform was probed with uHTS for biocatalysts anchored to yeast with enrichment close to the theoretically calculated limit and cell-to-cell interactions. MDE-FACS allowed the identification of human butyrylcholinesterase mutants that undergo self-reactivation after inhibition by the organophosphorus agent paraoxon. The versatility of the platform allowed the identification of bacteria, including slow-growing oral microbiota species that suppress the growth of a common pathogen, Staphylococcus aureus, and predicted which genera were associated with inhibitory activity.

Original languageEnglish (US)
Pages (from-to)2550-2555
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume114
Issue number10
DOIs
StatePublished - Mar 7 2017

Keywords

  • Butyrylcholinesterase
  • Cell-cell interactions
  • Microfluidic encapsulation
  • Staphylococcus aureus
  • Ultrahigh-throughput screening

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'Microfluidic droplet platform for ultrahigh-throughput single-cell screening of biodiversity'. Together they form a unique fingerprint.

  • Cite this

    Terekhov, S. S., Smirnov, I. V., Stepanova, A. V., Bobik, T. V., Mokrushina, Y. A., Ponomarenko, N. A., Belogurov, A. A., Rubtsova, M. P., Kartseva, O. V., Gomzikova, M. O., Moskovtsev, A. A., Bukatin, A. S., Dubina, M. V., Kostryukova, E. S., Babenko, V. V., Vakhitova, M. T., Manolov, A. I., Malakhova, M. V., Kornienko, M. A., ... Altman, S. (2017). Microfluidic droplet platform for ultrahigh-throughput single-cell screening of biodiversity. Proceedings of the National Academy of Sciences of the United States of America, 114(10), 2550-2555. https://doi.org/10.1073/pnas.1621226114