Abstract

A fluidic device capable of aspirating microparticles in aqueous medium and transporting them under controlled conditions to a chosen destination is reported. The device is fabricated through repeated stacking of aligned, laser cut acrylic, mylar and adhesive layers. The rectangular cross-section of the primary flow chamber within the device follows a specific mathematical function to achieve a desired velocity profile for improved particle tracking. Flow is controlled into and out of the chamber by three-way solenoid valves to allow changes in flow direction and to minimize capacitance in the fluidic circuit. The valves are connected to syringe pumps which generate positive and negative pressures within the device. Aspirated microparticles can be accurately transported by controlling the ON/OFF state of the solenoid valves. Computational fluid dynamic (CFD) models are employed to compare the theoretically derived velocity field and pressure distributions with analytical solutions and observations. Processed imagery showed the positions versus time of microparticle flowing through the chamber channel.

Original languageEnglish (US)
Title of host publicationASME International Mechanical Engineering Congress and Exposition, Proceedings
PublisherAmerican Society of Mechanical Engineers (ASME)
Pages2213-2220
Number of pages8
Volume9
EditionPART C
ISBN (Print)9780791843826
DOIs
StatePublished - 2010
EventASME 2009 International Mechanical Engineering Congress and Exposition, IMECE2009 - Lake Buena Vista, FL, United States
Duration: Nov 13 2009Nov 19 2009

Other

OtherASME 2009 International Mechanical Engineering Congress and Exposition, IMECE2009
CountryUnited States
CityLake Buena Vista, FL
Period11/13/0911/19/09

Fingerprint

Solenoid valves
Microfluidics
Fluidic devices
Syringes
Fluidics
Pressure distribution
Acrylics
Dynamic models
Adhesives
Computational fluid dynamics
Capacitance
Pumps
Networks (circuits)
Lasers

ASJC Scopus subject areas

  • Mechanical Engineering

Cite this

Merza, S. A., Posner, J. D., Anis, Y. H., Young, A. C., Johnson, R. H., & Meldrum, D. (2010). Microfluidic device for transport and observation of single cells. In ASME International Mechanical Engineering Congress and Exposition, Proceedings (PART C ed., Vol. 9, pp. 2213-2220). American Society of Mechanical Engineers (ASME). https://doi.org/10.1115/IMECE2009-13019

Microfluidic device for transport and observation of single cells. / Merza, Saeed A.; Posner, Jonathan D.; Anis, Yasser H.; Young, A. Cody; Johnson, Roger H.; Meldrum, Deirdre.

ASME International Mechanical Engineering Congress and Exposition, Proceedings. Vol. 9 PART C. ed. American Society of Mechanical Engineers (ASME), 2010. p. 2213-2220.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Merza, SA, Posner, JD, Anis, YH, Young, AC, Johnson, RH & Meldrum, D 2010, Microfluidic device for transport and observation of single cells. in ASME International Mechanical Engineering Congress and Exposition, Proceedings. PART C edn, vol. 9, American Society of Mechanical Engineers (ASME), pp. 2213-2220, ASME 2009 International Mechanical Engineering Congress and Exposition, IMECE2009, Lake Buena Vista, FL, United States, 11/13/09. https://doi.org/10.1115/IMECE2009-13019
Merza SA, Posner JD, Anis YH, Young AC, Johnson RH, Meldrum D. Microfluidic device for transport and observation of single cells. In ASME International Mechanical Engineering Congress and Exposition, Proceedings. PART C ed. Vol. 9. American Society of Mechanical Engineers (ASME). 2010. p. 2213-2220 https://doi.org/10.1115/IMECE2009-13019
Merza, Saeed A. ; Posner, Jonathan D. ; Anis, Yasser H. ; Young, A. Cody ; Johnson, Roger H. ; Meldrum, Deirdre. / Microfluidic device for transport and observation of single cells. ASME International Mechanical Engineering Congress and Exposition, Proceedings. Vol. 9 PART C. ed. American Society of Mechanical Engineers (ASME), 2010. pp. 2213-2220
@inproceedings{810f3d0293a34ebda0b02d1ac7efed2f,
title = "Microfluidic device for transport and observation of single cells",
abstract = "A fluidic device capable of aspirating microparticles in aqueous medium and transporting them under controlled conditions to a chosen destination is reported. The device is fabricated through repeated stacking of aligned, laser cut acrylic, mylar and adhesive layers. The rectangular cross-section of the primary flow chamber within the device follows a specific mathematical function to achieve a desired velocity profile for improved particle tracking. Flow is controlled into and out of the chamber by three-way solenoid valves to allow changes in flow direction and to minimize capacitance in the fluidic circuit. The valves are connected to syringe pumps which generate positive and negative pressures within the device. Aspirated microparticles can be accurately transported by controlling the ON/OFF state of the solenoid valves. Computational fluid dynamic (CFD) models are employed to compare the theoretically derived velocity field and pressure distributions with analytical solutions and observations. Processed imagery showed the positions versus time of microparticle flowing through the chamber channel.",
author = "Merza, {Saeed A.} and Posner, {Jonathan D.} and Anis, {Yasser H.} and Young, {A. Cody} and Johnson, {Roger H.} and Deirdre Meldrum",
year = "2010",
doi = "10.1115/IMECE2009-13019",
language = "English (US)",
isbn = "9780791843826",
volume = "9",
pages = "2213--2220",
booktitle = "ASME International Mechanical Engineering Congress and Exposition, Proceedings",
publisher = "American Society of Mechanical Engineers (ASME)",
edition = "PART C",

}

TY - GEN

T1 - Microfluidic device for transport and observation of single cells

AU - Merza, Saeed A.

AU - Posner, Jonathan D.

AU - Anis, Yasser H.

AU - Young, A. Cody

AU - Johnson, Roger H.

AU - Meldrum, Deirdre

PY - 2010

Y1 - 2010

N2 - A fluidic device capable of aspirating microparticles in aqueous medium and transporting them under controlled conditions to a chosen destination is reported. The device is fabricated through repeated stacking of aligned, laser cut acrylic, mylar and adhesive layers. The rectangular cross-section of the primary flow chamber within the device follows a specific mathematical function to achieve a desired velocity profile for improved particle tracking. Flow is controlled into and out of the chamber by three-way solenoid valves to allow changes in flow direction and to minimize capacitance in the fluidic circuit. The valves are connected to syringe pumps which generate positive and negative pressures within the device. Aspirated microparticles can be accurately transported by controlling the ON/OFF state of the solenoid valves. Computational fluid dynamic (CFD) models are employed to compare the theoretically derived velocity field and pressure distributions with analytical solutions and observations. Processed imagery showed the positions versus time of microparticle flowing through the chamber channel.

AB - A fluidic device capable of aspirating microparticles in aqueous medium and transporting them under controlled conditions to a chosen destination is reported. The device is fabricated through repeated stacking of aligned, laser cut acrylic, mylar and adhesive layers. The rectangular cross-section of the primary flow chamber within the device follows a specific mathematical function to achieve a desired velocity profile for improved particle tracking. Flow is controlled into and out of the chamber by three-way solenoid valves to allow changes in flow direction and to minimize capacitance in the fluidic circuit. The valves are connected to syringe pumps which generate positive and negative pressures within the device. Aspirated microparticles can be accurately transported by controlling the ON/OFF state of the solenoid valves. Computational fluid dynamic (CFD) models are employed to compare the theoretically derived velocity field and pressure distributions with analytical solutions and observations. Processed imagery showed the positions versus time of microparticle flowing through the chamber channel.

UR - http://www.scopus.com/inward/record.url?scp=77954296747&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77954296747&partnerID=8YFLogxK

U2 - 10.1115/IMECE2009-13019

DO - 10.1115/IMECE2009-13019

M3 - Conference contribution

AN - SCOPUS:77954296747

SN - 9780791843826

VL - 9

SP - 2213

EP - 2220

BT - ASME International Mechanical Engineering Congress and Exposition, Proceedings

PB - American Society of Mechanical Engineers (ASME)

ER -