Microelectrode array (MEA) platform for targeted neuronal transfection and recording

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13 Scopus citations

Abstract

Techniques used for nonviral gene transfection often have poor spatial resolution. In this letter, we present a microelectrode array (MEA) system that can precisely transfect exogenous molecules into targeted primary neurons using microelectroporation. An optimal cathodic pulse 4 V in amplitude and 1 ms in duration resulted in a transfection efficiency of 56% and a viability of 82%. Finally, siRNA molecules were transfected into targeted neurons in culture using the aforementioned system.

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Keywords

  • Biochip
  • Electroporation
  • siRNA
  • Transfection

ASJC Scopus subject areas

  • Biomedical Engineering

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