MicroED Sample Preparation and Data Collection For Protein Crystals

Guanhong Bu, Brent L. Nannenga

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Microcrystal Electron Diffraction (MicroED) enables structure determination of very small crystals that are much too small to be of use for other conventional diffraction techniques. MicroED has been used to determine the structures of many proteins and small organic molecules, and the technique can be performed on most standard cryo-TEM instruments equipped with high-speed detectors capable of collecting electron diffraction data. Here, we present protocols for MicroED sample preparation and data collection for protein microcrystals.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages287-297
Number of pages11
DOIs
StatePublished - 2021

Publication series

NameMethods in Molecular Biology
Volume2215
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • MicroED
  • Microcrystal electron diffraction
  • Protein crystallography
  • Sample preparation
  • Structural biology

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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