MicroED opens a new era for biological structure determination

Brent Nannenga, Tamir Gonen

Research output: Contribution to journalReview article

26 Scopus citations

Abstract

In 2013 we unveiled the cryo-electron microscopy (CryoEM) method of MicroED, or three-dimensional (3D) electron diffraction of microscopic crystals. Here tiny 3D crystals of biological material are used in an electron microscope for diffraction data collection under cryogenic conditions. The data is indexed, integrated, merged and scaled using standard X-ray crystallography software to determine structures at atomic resolution. In this review we provide an overview of the MicroED method and compare it with other CryoEM methods.

Original languageEnglish (US)
Pages (from-to)128-135
Number of pages8
JournalCurrent Opinion in Structural Biology
Volume40
DOIs
StatePublished - Oct 1 2016

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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