MicroED opens a new era for biological structure determination

Brent Nannenga; Tamir Gonen

doi:10.1016/j.sbi.2016.09.007

MicroED opens a new era for biological structure determination

Brent Nannenga, Tamir Gonen

Research output: Contribution to journal › Review article › peer-review

36 Scopus citations

Abstract

In 2013 we unveiled the cryo-electron microscopy (CryoEM) method of MicroED, or three-dimensional (3D) electron diffraction of microscopic crystals. Here tiny 3D crystals of biological material are used in an electron microscope for diffraction data collection under cryogenic conditions. The data is indexed, integrated, merged and scaled using standard X-ray crystallography software to determine structures at atomic resolution. In this review we provide an overview of the MicroED method and compare it with other CryoEM methods.

Original language	English (US)
Pages (from-to)	128-135
Number of pages	8
Journal	Current Opinion in Structural Biology
Volume	40
DOIs	https://doi.org/10.1016/j.sbi.2016.09.007
State	Published - Oct 1 2016

ASJC Scopus subject areas

Structural Biology
Molecular Biology

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10.1016/j.sbi.2016.09.007

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title = "MicroED opens a new era for biological structure determination",

abstract = "In 2013 we unveiled the cryo-electron microscopy (CryoEM) method of MicroED, or three-dimensional (3D) electron diffraction of microscopic crystals. Here tiny 3D crystals of biological material are used in an electron microscope for diffraction data collection under cryogenic conditions. The data is indexed, integrated, merged and scaled using standard X-ray crystallography software to determine structures at atomic resolution. In this review we provide an overview of the MicroED method and compare it with other CryoEM methods.",

author = "Brent Nannenga and Tamir Gonen",

note = "Funding Information: The Gonen laboratory is supported by the Howard Hughes Medical Institute. Publisher Copyright: {\textcopyright} 2016 Elsevier Ltd",

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AB - In 2013 we unveiled the cryo-electron microscopy (CryoEM) method of MicroED, or three-dimensional (3D) electron diffraction of microscopic crystals. Here tiny 3D crystals of biological material are used in an electron microscope for diffraction data collection under cryogenic conditions. The data is indexed, integrated, merged and scaled using standard X-ray crystallography software to determine structures at atomic resolution. In this review we provide an overview of the MicroED method and compare it with other CryoEM methods.

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MicroED opens a new era for biological structure determination

Abstract

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