MicroED data collection and processing

Johan Hattne, Francis E. Reyes, Brent L. Nannenga, Dan Shi, M. Jason De La Cruz, Andrew G.W. Leslie, Tamir Gonen

Research output: Contribution to journalArticle

51 Scopus citations

Abstract

MicroED, a method at the intersection of X-ray crystallography and electron cryo-microscopy, has rapidly progressed by exploiting advances in both fields and has already been successfully employed to determine the atomic structures of several proteins from sub-micron-sized, three-dimensional crystals. A major limiting factor in X-ray crystallography is the requirement for large and well ordered crystals. By permitting electron diffraction patterns to be collected from much smaller crystals, or even single well ordered domains of large crystals composed of several small mosaic blocks, MicroED has the potential to overcome the limiting size requirement and enable structural studies on difficult-to-crystallize samples. This communication details the steps for sample preparation, data collection and reduction necessary to obtain refined, high-resolution, three-dimensional models by MicroED, and presents some of its unique challenges.

Original languageEnglish (US)
Pages (from-to)353-360
Number of pages8
JournalActa Crystallographica Section A: Foundations and Advances
Volume71
DOIs
StatePublished - Jul 1 2015

Keywords

  • MicroED
  • cryo-EM
  • crystallography
  • electron diffraction
  • nanocrystals

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Materials Science(all)
  • Condensed Matter Physics
  • Physical and Theoretical Chemistry
  • Inorganic Chemistry

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  • Cite this

    Hattne, J., Reyes, F. E., Nannenga, B. L., Shi, D., De La Cruz, M. J., Leslie, A. G. W., & Gonen, T. (2015). MicroED data collection and processing. Acta Crystallographica Section A: Foundations and Advances, 71, 353-360. https://doi.org/10.1107/S2053273315010669