Microchannel networks for electrophoretic separations

Joël S. Rossier, Alexandra Ros, Frédéric Reymond, Rosaria Ferrigno, Françols Bianchi, Hubert H. Girault

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

UV excimer laser photoablation was used to micro-machine polymer substrates not only to drill microchannel structures but also to change the surface physical properties of the substrates. We first describe how UV laser photoablation can be used for the patterning of biomolecules on a polymer and discuss parameters such as surface coverage of active antibodies and equilibration time. Secondly, we show how to design a single-use capillary electrophoresis system comprising an on-chip injector, column and electrochemical detector. The potential of this disposable plastic device is discussed and briefly compared to classical systems. Finally, preliminary results on protein separation by isoelectric focusing on a disposable microchip are presented.

Original languageEnglish (US)
Title of host publicationFrom Genome to Proteome: Advances in the Practice & Application of Proteomics
PublisherWiley Blackwell
Pages147-151
Number of pages5
ISBN (Print)9783527613489, 9783527301546
DOIs
StatePublished - Dec 26 2007
Externally publishedYes

Keywords

  • Capillary electrophoresis
  • Disposable plastic microchip
  • Immunoassay
  • Photoablation
  • Protein separation

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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  • Cite this

    Rossier, J. S., Ros, A., Reymond, F., Ferrigno, R., Bianchi, F., & Girault, H. H. (2007). Microchannel networks for electrophoretic separations. In From Genome to Proteome: Advances in the Practice & Application of Proteomics (pp. 147-151). Wiley Blackwell. https://doi.org/10.1002/9783527613489.ch17