Microchannel networks for electrophoretic separations

Joël S. Rossier; Alexandra Schwarz; Frédéric Reymond; Rosaria Ferrigno; Françols Bianchi; Hubert H. Girault

doi:10.1002/9783527613489.ch17

Microchannel networks for electrophoretic separations

Joël S. Rossier, Alexandra Schwarz, Frédéric Reymond, Rosaria Ferrigno, Françols Bianchi, Hubert H. Girault

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

UV excimer laser photoablation was used to micro-machine polymer substrates not only to drill microchannel structures but also to change the surface physical properties of the substrates. We first describe how UV laser photoablation can be used for the patterning of biomolecules on a polymer and discuss parameters such as surface coverage of active antibodies and equilibration time. Secondly, we show how to design a single-use capillary electrophoresis system comprising an on-chip injector, column and electrochemical detector. The potential of this disposable plastic device is discussed and briefly compared to classical systems. Finally, preliminary results on protein separation by isoelectric focusing on a disposable microchip are presented.

Original language	English (US)
Title of host publication	From Genome to Proteome: Advances in the Practice & Application of Proteomics
Publisher	Wiley-Blackwell
Pages	147-151
Number of pages	5
ISBN (Electronic)	9783527613489
ISBN (Print)	9783527301546
DOIs	https://doi.org/10.1002/9783527613489.ch17
State	Published - Dec 26 2007
Externally published	Yes

Keywords

Capillary electrophoresis
Disposable plastic microchip
Immunoassay
Photoablation
Protein separation

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

Access to Document

10.1002/9783527613489.ch17

Cite this

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abstract = "UV excimer laser photoablation was used to micro-machine polymer substrates not only to drill microchannel structures but also to change the surface physical properties of the substrates. We first describe how UV laser photoablation can be used for the patterning of biomolecules on a polymer and discuss parameters such as surface coverage of active antibodies and equilibration time. Secondly, we show how to design a single-use capillary electrophoresis system comprising an on-chip injector, column and electrochemical detector. The potential of this disposable plastic device is discussed and briefly compared to classical systems. Finally, preliminary results on protein separation by isoelectric focusing on a disposable microchip are presented.",

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T1 - Microchannel networks for electrophoretic separations

AU - Rossier, Joël S.

AU - Schwarz, Alexandra

AU - Reymond, Frédéric

AU - Ferrigno, Rosaria

AU - Bianchi, Françols

AU - Girault, Hubert H.

PY - 2007/12/26

Y1 - 2007/12/26

N2 - UV excimer laser photoablation was used to micro-machine polymer substrates not only to drill microchannel structures but also to change the surface physical properties of the substrates. We first describe how UV laser photoablation can be used for the patterning of biomolecules on a polymer and discuss parameters such as surface coverage of active antibodies and equilibration time. Secondly, we show how to design a single-use capillary electrophoresis system comprising an on-chip injector, column and electrochemical detector. The potential of this disposable plastic device is discussed and briefly compared to classical systems. Finally, preliminary results on protein separation by isoelectric focusing on a disposable microchip are presented.

AB - UV excimer laser photoablation was used to micro-machine polymer substrates not only to drill microchannel structures but also to change the surface physical properties of the substrates. We first describe how UV laser photoablation can be used for the patterning of biomolecules on a polymer and discuss parameters such as surface coverage of active antibodies and equilibration time. Secondly, we show how to design a single-use capillary electrophoresis system comprising an on-chip injector, column and electrochemical detector. The potential of this disposable plastic device is discussed and briefly compared to classical systems. Finally, preliminary results on protein separation by isoelectric focusing on a disposable microchip are presented.

KW - Capillary electrophoresis

KW - Disposable plastic microchip

KW - Immunoassay

KW - Photoablation

KW - Protein separation

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M3 - Chapter

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BT - From Genome to Proteome: Advances in the Practice & Application of Proteomics

PB - Wiley-Blackwell

ER -

Microchannel networks for electrophoretic separations

Abstract

Keywords

ASJC Scopus subject areas

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