Microchannel networks for electrophoretic separations

J. S. Rossier, A. Schwarz, F. Reymond, R. Ferrigno, F. Bianchi, H. H. Girault

Research output: Contribution to journalArticlepeer-review

100 Scopus citations

Abstract

UV excimer laser photoablation was used to micro-machine polymer substrates not only to drill microchannel structures but also to change the surface physical properties of the substrates. We first describe how UV laser photoablation can be used for the patterning of biomolecules on a polymer and discuss parameters such as surface coverage of active antibodies and equilibration time. Secondly, we show how to design a single-use capillary electrophoresis system comprising an on-chip injector, column and electrochemical detector. The potential of this disposable plastic device is discussed and briefly compared to classical systems. Finally, preliminary results on protein separation by isoelectric focusing on a disposable microchip are presented.

Original languageEnglish (US)
Pages (from-to)727-731
Number of pages5
JournalElectrophoresis
Volume20
Issue number4-5
DOIs
StatePublished - May 22 1999
Externally publishedYes

Keywords

  • Capillary electrophoresis
  • Disposable plastic microchip
  • Immunoassay
  • Photoablation
  • Protein separation

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

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