Microbial energetics and stoichiometry for biodegradation of aromatic compounds involving oxygenation reactions

Seung H. Woo, Bruce Rittmann

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Oxygenation reactions significantly alter the energy and electron flows and, consequently, the overall stoichiometry for the microbial utilization of aromatic compounds. Oxygenation reactions do not yield a net release of electrons, but require an input of electrons to reduce oxygen molecules. The biodegradation pathway of phenanthrene as a model compound was analyzed to determine the impact of oxygenation reactions on overall stoichiometry using the half-reaction method. For individual oxygenation reactions, the half-reaction method for analyzing the electron and energy flows must be modified, because the reactions do not release electrons for synthesis or energy generation. Coupling the oxygenation reaction to subsequent reaction steps provides a net electron release for the coupled reactions. Modeling results indicate that oxygenation reactions increase the oxygen requirement and reduce the cell yield, compared to the conventional mineralization represented by hydroxylation reactions in place of oxygenations. The computed yields considering oxygenation reactions conform better to empirical yields reported in the literature than do yields computed by the hydroxylation single-step methods. The coupled-reaction model also is consistent with information about the ways in which micro-organisms that degrade aromatics accumulate intermediates, regulate degradation genes, and organize enzyme clusters.

Original languageEnglish (US)
Pages (from-to)213-227
Number of pages15
JournalBiodegradation
Volume11
Issue number4
DOIs
StatePublished - 2000
Externally publishedYes

Fingerprint

Oxygenation
Aromatic compounds
oxygenation
stoichiometry
Biodegradation
Stoichiometry
biodegradation
energetics
Electrons
electron
Hydroxylation
Oxygen
oxygen
aromatic compound
energy flow
phenanthrene
energy
Enzymes
Genes
mineralization

Keywords

  • Aromatics biodegradation
  • Energetics
  • Intermediates
  • Oxygenation
  • Phenanthrene
  • Stoichio metry
  • Yield

ASJC Scopus subject areas

  • Biotechnology

Cite this

Microbial energetics and stoichiometry for biodegradation of aromatic compounds involving oxygenation reactions. / Woo, Seung H.; Rittmann, Bruce.

In: Biodegradation, Vol. 11, No. 4, 2000, p. 213-227.

Research output: Contribution to journalArticle

@article{442477d4138c41dc9e47b3480195185e,
title = "Microbial energetics and stoichiometry for biodegradation of aromatic compounds involving oxygenation reactions",
abstract = "Oxygenation reactions significantly alter the energy and electron flows and, consequently, the overall stoichiometry for the microbial utilization of aromatic compounds. Oxygenation reactions do not yield a net release of electrons, but require an input of electrons to reduce oxygen molecules. The biodegradation pathway of phenanthrene as a model compound was analyzed to determine the impact of oxygenation reactions on overall stoichiometry using the half-reaction method. For individual oxygenation reactions, the half-reaction method for analyzing the electron and energy flows must be modified, because the reactions do not release electrons for synthesis or energy generation. Coupling the oxygenation reaction to subsequent reaction steps provides a net electron release for the coupled reactions. Modeling results indicate that oxygenation reactions increase the oxygen requirement and reduce the cell yield, compared to the conventional mineralization represented by hydroxylation reactions in place of oxygenations. The computed yields considering oxygenation reactions conform better to empirical yields reported in the literature than do yields computed by the hydroxylation single-step methods. The coupled-reaction model also is consistent with information about the ways in which micro-organisms that degrade aromatics accumulate intermediates, regulate degradation genes, and organize enzyme clusters.",
keywords = "Aromatics biodegradation, Energetics, Intermediates, Oxygenation, Phenanthrene, Stoichio metry, Yield",
author = "Woo, {Seung H.} and Bruce Rittmann",
year = "2000",
doi = "10.1023/A:1011162830401",
language = "English (US)",
volume = "11",
pages = "213--227",
journal = "Biodegradation",
issn = "0923-9820",
publisher = "Springer Netherlands",
number = "4",

}

TY - JOUR

T1 - Microbial energetics and stoichiometry for biodegradation of aromatic compounds involving oxygenation reactions

AU - Woo, Seung H.

AU - Rittmann, Bruce

PY - 2000

Y1 - 2000

N2 - Oxygenation reactions significantly alter the energy and electron flows and, consequently, the overall stoichiometry for the microbial utilization of aromatic compounds. Oxygenation reactions do not yield a net release of electrons, but require an input of electrons to reduce oxygen molecules. The biodegradation pathway of phenanthrene as a model compound was analyzed to determine the impact of oxygenation reactions on overall stoichiometry using the half-reaction method. For individual oxygenation reactions, the half-reaction method for analyzing the electron and energy flows must be modified, because the reactions do not release electrons for synthesis or energy generation. Coupling the oxygenation reaction to subsequent reaction steps provides a net electron release for the coupled reactions. Modeling results indicate that oxygenation reactions increase the oxygen requirement and reduce the cell yield, compared to the conventional mineralization represented by hydroxylation reactions in place of oxygenations. The computed yields considering oxygenation reactions conform better to empirical yields reported in the literature than do yields computed by the hydroxylation single-step methods. The coupled-reaction model also is consistent with information about the ways in which micro-organisms that degrade aromatics accumulate intermediates, regulate degradation genes, and organize enzyme clusters.

AB - Oxygenation reactions significantly alter the energy and electron flows and, consequently, the overall stoichiometry for the microbial utilization of aromatic compounds. Oxygenation reactions do not yield a net release of electrons, but require an input of electrons to reduce oxygen molecules. The biodegradation pathway of phenanthrene as a model compound was analyzed to determine the impact of oxygenation reactions on overall stoichiometry using the half-reaction method. For individual oxygenation reactions, the half-reaction method for analyzing the electron and energy flows must be modified, because the reactions do not release electrons for synthesis or energy generation. Coupling the oxygenation reaction to subsequent reaction steps provides a net electron release for the coupled reactions. Modeling results indicate that oxygenation reactions increase the oxygen requirement and reduce the cell yield, compared to the conventional mineralization represented by hydroxylation reactions in place of oxygenations. The computed yields considering oxygenation reactions conform better to empirical yields reported in the literature than do yields computed by the hydroxylation single-step methods. The coupled-reaction model also is consistent with information about the ways in which micro-organisms that degrade aromatics accumulate intermediates, regulate degradation genes, and organize enzyme clusters.

KW - Aromatics biodegradation

KW - Energetics

KW - Intermediates

KW - Oxygenation

KW - Phenanthrene

KW - Stoichio metry

KW - Yield

UR - http://www.scopus.com/inward/record.url?scp=0034439467&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034439467&partnerID=8YFLogxK

U2 - 10.1023/A:1011162830401

DO - 10.1023/A:1011162830401

M3 - Article

C2 - 11432580

AN - SCOPUS:0034439467

VL - 11

SP - 213

EP - 227

JO - Biodegradation

JF - Biodegradation

SN - 0923-9820

IS - 4

ER -