Microbial ecology of a perchlorate-reducing, hydrogen-based membrane biofilm reactor

Robert Nerenberg, Yasunori Kawagoshi, Bruce Rittmann

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

The hydrogen-based membrane biofilm reactor (MBfR) has been shown to reduce perchlorate to below 4 μg/L, but little is known about the microbial ecology of this or other hydrogen-based reactors, especially when influent perchlorate concentrations are much lower than the influent oxygen and nitrate concentrations. Dissimilatory (per)chlorate-reducing bacteria (PCRB) can use oxygen as an electron acceptor, and most can also use nitrate. Since oxygen and nitrate can be reduced concurrently with perchlorate, they may serve as primary electron acceptors, sustaining PCRB when the perchlorate concentrations are very low. We studied five identical MBfRs, all seeded with the same inoculum and initially supplied with oxygen, or oxygen plus nitrate, in the influent. After 20 days, perchlorate was added to four MBfRs at influent concentrations of 100-10,000 μg/L, while the fifth was maintained as a control. One day after perchlorate addition, the MBfRs displayed limited perchlorate reduction, suggesting a low initial abundance of PCRB. However, perchlorate reduction improved significantly over time, and denaturing gradient gel electrophoresis (DGGE) analyses suggested an increasing abundance of a single Dechloromonas species. Fluorescence in-situ hybridization (FISH) tests showed that the Dechloromonas species accounted for 14% of the bacterial count in the control MBfR, and 22%, 31%, and 49% in the MBfRs receiving nitrate plus 100, 1000, and 10,000 μg/L perchlorate, respectively. The abundance was 34% in the MBfR receiving oxygen plus 1000 μg/L perchlorate. These results suggest that oxygen is more favorable than nitrate as a primary electron acceptor for PCRB, that PCRB are present at low levels even without perchlorate, and that the presence of perchlorate, even at low levels relative to nitrate or oxygen, significantly enhances selection for PCRB.

Original languageEnglish (US)
Pages (from-to)1151-1159
Number of pages9
JournalWater Research
Volume42
Issue number4-5
DOIs
StatePublished - Feb 2008

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microbial ecology
perchlorate
Biofilms
Ecology
biofilm
Nitrates
hydrogen
membrane
Membranes
Bacteria
Hydrogen
Oxygen
oxygen
nitrate
bacterium
Electrons
electron
reactor
Electrophoresis
Gels

Keywords

  • Hydrogen
  • Membrane biofilm reactor
  • Microbial ecology
  • Perchlorate
  • Secondary substrate

ASJC Scopus subject areas

  • Earth-Surface Processes

Cite this

Microbial ecology of a perchlorate-reducing, hydrogen-based membrane biofilm reactor. / Nerenberg, Robert; Kawagoshi, Yasunori; Rittmann, Bruce.

In: Water Research, Vol. 42, No. 4-5, 02.2008, p. 1151-1159.

Research output: Contribution to journalArticle

Nerenberg, Robert ; Kawagoshi, Yasunori ; Rittmann, Bruce. / Microbial ecology of a perchlorate-reducing, hydrogen-based membrane biofilm reactor. In: Water Research. 2008 ; Vol. 42, No. 4-5. pp. 1151-1159.
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