Microbial community structure of ethanol type fermentation in bio-hydrogen production

Nanqi Ren, Defeng Xing, Bruce Rittmann, Lihua Zhao, Tianhui Xie, Xin Zhao

Research output: Contribution to journalArticle

148 Citations (Scopus)

Abstract

Three continuous stirred-tank reactors (CSTRs) were used for H2 production from molasses wastewater at influent pH of 6.0-6.5 (reactor A), 5.5-6.0 (reactor B), or 4.0-4.5 (reactor C). After operation for 28 days, the microbial community formed ethanol type (C), propionate type (A) and ethanol-butyrate-mixed type (B) fermentation. The H2 production rate was the highest for ethanol type fermentation, 0.40 l (g VSS)-1 day-1 or 0.45 l H2 (g COD removed)-1. Microbial community dynamics and diversity were analysed using double-gradient denaturing gradient gel electrophoresis (DG-DGGE). Denaturing gradient gel electrophoresis profiles indicated that the community structures changed quickly in the first 14 days. Phylogenetic analysis indicated that the dominant bacterial groups were low G+C Gram-positive bacteria, Bacteroides, γ-Proteobacteria and Actinobacteria; α-Proteobacteria, β-Proteobacteria, δ-Proteobacteria and Spirochaetes were also presented as minor groups in the three reactors. H2-producing bacteria were affiliated with Ethanoligenens, Acetanaerobacterium, Clostridium, Megasphaera, Citrobacter and Bacteroides. An ethanol-based H2-producing bacterium, Ethanoligenens harbinense CGMCC1152, was isolated from reactor C and visualized using fluorescence in situ hybridization (FISH) to be 19% of the eubacteria in reactor C. In addition, isoenzyme activity staining for alcohol dehydrogenase (ADH) supported that the majority of ethanol-producing bacteria were affiliated with Ethanoligenens in the microbial community.

Original languageEnglish (US)
Pages (from-to)1112-1125
Number of pages14
JournalEnvironmental Microbiology
Volume9
Issue number5
DOIs
StatePublished - May 2007

Fingerprint

hydrogen production
Proteobacteria
Fermentation
fermentation
microbial communities
microbial community
Hydrogen
ethanol
community structure
Ethanol
hydrogen
Bacteria
Denaturing Gradient Gel Electrophoresis
Bacteroides
denaturing gradient gel electrophoresis
bacterium
bacteria
Megasphaera
Citrobacter
Molasses

ASJC Scopus subject areas

  • Environmental Science(all)
  • Microbiology
  • Applied Microbiology and Biotechnology

Cite this

Microbial community structure of ethanol type fermentation in bio-hydrogen production. / Ren, Nanqi; Xing, Defeng; Rittmann, Bruce; Zhao, Lihua; Xie, Tianhui; Zhao, Xin.

In: Environmental Microbiology, Vol. 9, No. 5, 05.2007, p. 1112-1125.

Research output: Contribution to journalArticle

Ren, Nanqi ; Xing, Defeng ; Rittmann, Bruce ; Zhao, Lihua ; Xie, Tianhui ; Zhao, Xin. / Microbial community structure of ethanol type fermentation in bio-hydrogen production. In: Environmental Microbiology. 2007 ; Vol. 9, No. 5. pp. 1112-1125.
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