TY - JOUR
T1 - Microbial community structure of ethanol type fermentation in bio-hydrogen production
AU - Ren, Nanqi
AU - Xing, Defeng
AU - Rittmann, Bruce
AU - Zhao, Lihua
AU - Xie, Tianhui
AU - Zhao, Xin
PY - 2007/5
Y1 - 2007/5
N2 - Three continuous stirred-tank reactors (CSTRs) were used for H2 production from molasses wastewater at influent pH of 6.0-6.5 (reactor A), 5.5-6.0 (reactor B), or 4.0-4.5 (reactor C). After operation for 28 days, the microbial community formed ethanol type (C), propionate type (A) and ethanol-butyrate-mixed type (B) fermentation. The H2 production rate was the highest for ethanol type fermentation, 0.40 l (g VSS)-1 day-1 or 0.45 l H2 (g COD removed)-1. Microbial community dynamics and diversity were analysed using double-gradient denaturing gradient gel electrophoresis (DG-DGGE). Denaturing gradient gel electrophoresis profiles indicated that the community structures changed quickly in the first 14 days. Phylogenetic analysis indicated that the dominant bacterial groups were low G+C Gram-positive bacteria, Bacteroides, γ-Proteobacteria and Actinobacteria; α-Proteobacteria, β-Proteobacteria, δ-Proteobacteria and Spirochaetes were also presented as minor groups in the three reactors. H2-producing bacteria were affiliated with Ethanoligenens, Acetanaerobacterium, Clostridium, Megasphaera, Citrobacter and Bacteroides. An ethanol-based H2-producing bacterium, Ethanoligenens harbinense CGMCC1152, was isolated from reactor C and visualized using fluorescence in situ hybridization (FISH) to be 19% of the eubacteria in reactor C. In addition, isoenzyme activity staining for alcohol dehydrogenase (ADH) supported that the majority of ethanol-producing bacteria were affiliated with Ethanoligenens in the microbial community.
AB - Three continuous stirred-tank reactors (CSTRs) were used for H2 production from molasses wastewater at influent pH of 6.0-6.5 (reactor A), 5.5-6.0 (reactor B), or 4.0-4.5 (reactor C). After operation for 28 days, the microbial community formed ethanol type (C), propionate type (A) and ethanol-butyrate-mixed type (B) fermentation. The H2 production rate was the highest for ethanol type fermentation, 0.40 l (g VSS)-1 day-1 or 0.45 l H2 (g COD removed)-1. Microbial community dynamics and diversity were analysed using double-gradient denaturing gradient gel electrophoresis (DG-DGGE). Denaturing gradient gel electrophoresis profiles indicated that the community structures changed quickly in the first 14 days. Phylogenetic analysis indicated that the dominant bacterial groups were low G+C Gram-positive bacteria, Bacteroides, γ-Proteobacteria and Actinobacteria; α-Proteobacteria, β-Proteobacteria, δ-Proteobacteria and Spirochaetes were also presented as minor groups in the three reactors. H2-producing bacteria were affiliated with Ethanoligenens, Acetanaerobacterium, Clostridium, Megasphaera, Citrobacter and Bacteroides. An ethanol-based H2-producing bacterium, Ethanoligenens harbinense CGMCC1152, was isolated from reactor C and visualized using fluorescence in situ hybridization (FISH) to be 19% of the eubacteria in reactor C. In addition, isoenzyme activity staining for alcohol dehydrogenase (ADH) supported that the majority of ethanol-producing bacteria were affiliated with Ethanoligenens in the microbial community.
UR - http://www.scopus.com/inward/record.url?scp=34247850939&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34247850939&partnerID=8YFLogxK
U2 - 10.1111/j.1462-2920.2006.01234.x
DO - 10.1111/j.1462-2920.2006.01234.x
M3 - Article
C2 - 17472628
AN - SCOPUS:34247850939
SN - 1462-2912
VL - 9
SP - 1112
EP - 1125
JO - Environmental microbiology
JF - Environmental microbiology
IS - 5
ER -