Microbial community structure during nitrate and perchlorate reduction in ion-exchange brine using the hydrogen-based membrane biofilm reactor (MBfR)

Steven W. Van Ginkel, Regina Lamendella, William P. Kovacik, Jorge W. Santo Domingo, Bruce Rittmann

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Detoxification of perchlorate by microbial communities under denitrifying conditions has been recently reported, although the identity of the mixed populations involved in perchlorate reduction is not well understood. In order to address this, the bacterial diversity of membrane biofilm reactors (MBfR) set up under autotrophic denitrifying and perchlorate-reducing conditions were examined by analyses of the 16S rRNA gene sequences of clone libraries. Inocula from diverse locations were tested for their ability to reduce nitrate and perchlorate in synthetic ion exchange spent brine (45 g/l NaCl) using H2-based MBfRs. Phylogenetic analysis of 16S rRNA gene sequences showed that proteobacterial species dominated the biofilm communities, particularly nitrate-reducing γ-proteobacteria. Even though the inocula to the MBfRs came from different sources, clones closely related to Marinobacter hydrocarbonoclasticus represented 53% of all clones in the MBfR biofilms. The clone libraries contained no known perchlorate-reducing bacteria, which suggest that denitrifiers carried out perchlorate reduction, probably by secondary-utilization.

Original languageEnglish (US)
Pages (from-to)3747-3750
Number of pages4
JournalBioresource Technology
Volume101
Issue number10
DOIs
StatePublished - May 2010

Fingerprint

perchlorate
Biofilms
Nitrates
brine
biofilm
microbial community
Hydrogen
ion exchange
Ion exchange
community structure
hydrogen
nitrate
membrane
Membranes
clone
Genes
Detoxification
Bacteria
gene
detoxification

Keywords

  • Ion-exchange brine
  • Membrane biofilm reactor (MBfR)
  • Microbial ecology
  • Nitrate
  • Perchlorate

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Waste Management and Disposal

Cite this

Microbial community structure during nitrate and perchlorate reduction in ion-exchange brine using the hydrogen-based membrane biofilm reactor (MBfR). / Van Ginkel, Steven W.; Lamendella, Regina; Kovacik, William P.; Santo Domingo, Jorge W.; Rittmann, Bruce.

In: Bioresource Technology, Vol. 101, No. 10, 05.2010, p. 3747-3750.

Research output: Contribution to journalArticle

Van Ginkel, Steven W. ; Lamendella, Regina ; Kovacik, William P. ; Santo Domingo, Jorge W. ; Rittmann, Bruce. / Microbial community structure during nitrate and perchlorate reduction in ion-exchange brine using the hydrogen-based membrane biofilm reactor (MBfR). In: Bioresource Technology. 2010 ; Vol. 101, No. 10. pp. 3747-3750.
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