Microbial Chain Elongation and Subsequent Fermentation of Elongated Carboxylates as H2-Producing Processes for Sustained Reductive Dechlorination of Chlorinated Ethenes

Aide Robles, Theodora L. Yellowman, Sayalee Joshi, Srivatsan Mohana Rangan, Anca G. Delgado

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

In situ anaerobic groundwater bioremediation of trichloroethene (TCE) to nontoxic ethene is contingent on organohalide-respiring Dehalococcoidia, the most common strictly hydrogenotrophic Dehalococcoides mccartyi (D. mccartyi). The H2 requirement for D. mccartyi is fulfilled by adding various organic substrates (e.g., lactate, emulsified vegetable oil, and glucose/molasses), which require fermenting microorganisms to convert them to H2. The net flux of H2 is a crucial controlling parameter in the efficacy of bioremediation. H2 consumption by competing microorganisms (e.g., methanogens and homoacetogens) can diminish the rates of reductive dechlorination or stall the process altogether. Furthermore, some fermentation pathways do not produce H2 or having H2 as a product is not always thermodynamically favorable under environmental conditions. Here, we report on a novel application of microbial chain elongation as a H2-producing process for reductive dechlorination. In soil microcosms bioaugmented with dechlorinating and chain-elongating enrichment cultures, near stoichiometric conversion of TCE (0.07 ± 0.01, 0.60 ± 0.03, and 1.50 ± 0.20 mmol L-1 added sequentially) to ethene was achieved when initially stimulated by chain elongation of acetate and ethanol. Chain elongation initiated reductive dechlorination by liberating H2 in the conversion of acetate and ethanol to butyrate and caproate. Syntrophic fermentation of butyrate, a chain-elongation product, to H2 and acetate further sustained the reductive dechlorination activity. Methanogenesis was limited during TCE dechlorination in soil microcosms and absent in transfer cultures fed with chain-elongation substrates. This study provides critical fundamental knowledge toward the feasibility of chlorinated solvent bioremediation based on microbial chain elongation.

Original languageEnglish (US)
Pages (from-to)10398-10410
Number of pages13
JournalEnvironmental Science and Technology
Volume55
Issue number15
DOIs
StatePublished - Aug 3 2021

Keywords

  • butyrate fermentation
  • Clostridium kluyveri
  • Dehalococcoides mccartyi
  • groundwater bioremediation
  • medium-chain carboxylates
  • microbial chain elongation
  • reductive dechlorination
  • trichloroethene

ASJC Scopus subject areas

  • Chemistry(all)
  • Environmental Chemistry

Fingerprint

Dive into the research topics of 'Microbial Chain Elongation and Subsequent Fermentation of Elongated Carboxylates as H<sub>2</sub>-Producing Processes for Sustained Reductive Dechlorination of Chlorinated Ethenes'. Together they form a unique fingerprint.

Cite this