Microarray detection of E2F pathway activation and other targets in multiple sclerosis peripheral blood mononuclear cells

Antonio H. Iglesias; Sandra Camelo; Daehee Hwang; Raul Villanueva; George Stephanopoulos; Fernando Dangond

doi:10.1016/j.jneuroim.2004.01.017

Microarray detection of E2F pathway activation and other targets in multiple sclerosis peripheral blood mononuclear cells

Antonio H. Iglesias, Sandra Camelo, Daehee Hwang, Raul Villanueva, George Stephanopoulos, Fernando Dangond

Research output: Contribution to journal › Article › peer-review

59 Scopus citations

Abstract

We performed microarray analysis of peripheral blood mononuclear cells (PBMCs) from multiple sclerosis (MS) patients and detected a profile of immune cell activation, autoantigen upregulation, and enhanced E2F pathway transcription. Accordingly, E2f1-deficient mice manifested only mild disability upon induction of experimental autoimmune encephalomyelitis (EAE). Furthermore, PBMCs from Avonex-treated patients had lower expression of E2F targets. The profile was enriched in genes known to harbor MS-associated polymorphisms, or localized to MS susceptibility chromosomal regions. Our study shows that PBMC microarrays reflect MS pathobiology that can be validated in the EAE model.

Original language	English (US)
Pages (from-to)	163-177
Number of pages	15
Journal	Journal of Neuroimmunology
Volume	150
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jneuroim.2004.01.017
State	Published - May 2004
Externally published	Yes

Keywords

Autoimmunity
DNA microarrays
Experimental autoimmune encephalomyelitis
Gene expression
Multiple sclerosis

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Neurology
Clinical Neurology

Access to Document

10.1016/j.jneuroim.2004.01.017

Cite this

@article{c43303c1112040389b92288424a26453,

title = "Microarray detection of E2F pathway activation and other targets in multiple sclerosis peripheral blood mononuclear cells",

abstract = "We performed microarray analysis of peripheral blood mononuclear cells (PBMCs) from multiple sclerosis (MS) patients and detected a profile of immune cell activation, autoantigen upregulation, and enhanced E2F pathway transcription. Accordingly, E2f1-deficient mice manifested only mild disability upon induction of experimental autoimmune encephalomyelitis (EAE). Furthermore, PBMCs from Avonex-treated patients had lower expression of E2F targets. The profile was enriched in genes known to harbor MS-associated polymorphisms, or localized to MS susceptibility chromosomal regions. Our study shows that PBMC microarrays reflect MS pathobiology that can be validated in the EAE model.",

keywords = "Autoimmunity, DNA microarrays, Experimental autoimmune encephalomyelitis, Gene expression, Multiple sclerosis",

author = "Iglesias, {Antonio H.} and Sandra Camelo and Daehee Hwang and Raul Villanueva and George Stephanopoulos and Fernando Dangond",

note = "Funding Information: This work was funded by NIH R21 NS41623 (F.D.).",

year = "2004",

month = may,

doi = "10.1016/j.jneuroim.2004.01.017",

language = "English (US)",

volume = "150",

pages = "163--177",

journal = "Journal of Neuroimmunology",

issn = "0165-5728",

publisher = "Elsevier",

number = "1-2",

}

TY - JOUR

T1 - Microarray detection of E2F pathway activation and other targets in multiple sclerosis peripheral blood mononuclear cells

AU - Iglesias, Antonio H.

AU - Camelo, Sandra

AU - Hwang, Daehee

AU - Villanueva, Raul

AU - Stephanopoulos, George

AU - Dangond, Fernando

N1 - Funding Information: This work was funded by NIH R21 NS41623 (F.D.).

PY - 2004/5

Y1 - 2004/5

N2 - We performed microarray analysis of peripheral blood mononuclear cells (PBMCs) from multiple sclerosis (MS) patients and detected a profile of immune cell activation, autoantigen upregulation, and enhanced E2F pathway transcription. Accordingly, E2f1-deficient mice manifested only mild disability upon induction of experimental autoimmune encephalomyelitis (EAE). Furthermore, PBMCs from Avonex-treated patients had lower expression of E2F targets. The profile was enriched in genes known to harbor MS-associated polymorphisms, or localized to MS susceptibility chromosomal regions. Our study shows that PBMC microarrays reflect MS pathobiology that can be validated in the EAE model.

AB - We performed microarray analysis of peripheral blood mononuclear cells (PBMCs) from multiple sclerosis (MS) patients and detected a profile of immune cell activation, autoantigen upregulation, and enhanced E2F pathway transcription. Accordingly, E2f1-deficient mice manifested only mild disability upon induction of experimental autoimmune encephalomyelitis (EAE). Furthermore, PBMCs from Avonex-treated patients had lower expression of E2F targets. The profile was enriched in genes known to harbor MS-associated polymorphisms, or localized to MS susceptibility chromosomal regions. Our study shows that PBMC microarrays reflect MS pathobiology that can be validated in the EAE model.

KW - Autoimmunity

KW - DNA microarrays

KW - Experimental autoimmune encephalomyelitis

KW - Gene expression

KW - Multiple sclerosis

UR - http://www.scopus.com/inward/record.url?scp=1842687455&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1842687455&partnerID=8YFLogxK

U2 - 10.1016/j.jneuroim.2004.01.017

DO - 10.1016/j.jneuroim.2004.01.017

M3 - Article

C2 - 15081262

AN - SCOPUS:1842687455

SN - 0165-5728

VL - 150

SP - 163

EP - 177

JO - Journal of Neuroimmunology

JF - Journal of Neuroimmunology

IS - 1-2

ER -

Microarray detection of E2F pathway activation and other targets in multiple sclerosis peripheral blood mononuclear cells

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this