Microarray detection of E2F pathway activation and other targets in multiple sclerosis peripheral blood mononuclear cells

Antonio H. Iglesias, Sandra Camelo, Daehee Hwang, Raul Villanueva, George Stephanopoulos, Fernando Dangond

Research output: Contribution to journalArticle

57 Scopus citations

Abstract

We performed microarray analysis of peripheral blood mononuclear cells (PBMCs) from multiple sclerosis (MS) patients and detected a profile of immune cell activation, autoantigen upregulation, and enhanced E2F pathway transcription. Accordingly, E2f1-deficient mice manifested only mild disability upon induction of experimental autoimmune encephalomyelitis (EAE). Furthermore, PBMCs from Avonex-treated patients had lower expression of E2F targets. The profile was enriched in genes known to harbor MS-associated polymorphisms, or localized to MS susceptibility chromosomal regions. Our study shows that PBMC microarrays reflect MS pathobiology that can be validated in the EAE model.

Original languageEnglish (US)
Pages (from-to)163-177
Number of pages15
JournalJournal of Neuroimmunology
Volume150
Issue number1-2
DOIs
StatePublished - May 1 2004
Externally publishedYes

Keywords

  • Autoimmunity
  • DNA microarrays
  • Experimental autoimmune encephalomyelitis
  • Gene expression
  • Multiple sclerosis

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Neurology
  • Clinical Neurology

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