Methods to manipulate and monitor wnt signaling in human pluripotent stem cells

Ian J. Huggins, David Brafman, Karl Willert

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

Human pluripotent stem cells (hPSCs) may revolutionize medical practice by providing: (a) a renewable source of cells for tissue replacement therapies, (b) a powerful system to model human diseases in a dish, and (c) a platform for examining efficacy and safety of novel drugs. Furthermore, these cells offer a unique opportunity to study early human development in vitro, in particular, the process by which a seemingly uniform cell population interacts to give rise to the three main embryonic lineages: ectoderm, endoderm. and mesoderm. This process of lineage allocation is regulated by a number of inductive signals that are mediated by growth factors, including FGF, TGFβ, and Wnt. In this book chapter, we introduce a set of tools, methods, and protocols to specifically manipulate the Wnt signaling pathway with the intention of altering the cell fate outcome of hPSCs.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages161-181
Number of pages21
Volume1481
DOIs
StatePublished - 2016

Publication series

NameMethods in Molecular Biology
Volume1481
ISSN (Print)10643745

Fingerprint

Pluripotent Stem Cells
Endoderm
Ectoderm
Wnt Signaling Pathway
Human Development
Mesoderm
Cell- and Tissue-Based Therapy
Intercellular Signaling Peptides and Proteins
Safety
Pharmaceutical Preparations
Population

Keywords

  • Embryonic development
  • Human embryonic stem cell (hESC)
  • Human pluripotent stem cell (hPSC)
  • Induced pluripotent stem cell (iPSC)
  • Wnt

ASJC Scopus subject areas

  • Medicine(all)
  • Molecular Biology
  • Genetics

Cite this

Huggins, I. J., Brafman, D., & Willert, K. (2016). Methods to manipulate and monitor wnt signaling in human pluripotent stem cells. In Methods in Molecular Biology (Vol. 1481, pp. 161-181). (Methods in Molecular Biology; Vol. 1481). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6393-5_16

Methods to manipulate and monitor wnt signaling in human pluripotent stem cells. / Huggins, Ian J.; Brafman, David; Willert, Karl.

Methods in Molecular Biology. Vol. 1481 Humana Press Inc., 2016. p. 161-181 (Methods in Molecular Biology; Vol. 1481).

Research output: Chapter in Book/Report/Conference proceedingChapter

Huggins, IJ, Brafman, D & Willert, K 2016, Methods to manipulate and monitor wnt signaling in human pluripotent stem cells. in Methods in Molecular Biology. vol. 1481, Methods in Molecular Biology, vol. 1481, Humana Press Inc., pp. 161-181. https://doi.org/10.1007/978-1-4939-6393-5_16
Huggins IJ, Brafman D, Willert K. Methods to manipulate and monitor wnt signaling in human pluripotent stem cells. In Methods in Molecular Biology. Vol. 1481. Humana Press Inc. 2016. p. 161-181. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-6393-5_16
Huggins, Ian J. ; Brafman, David ; Willert, Karl. / Methods to manipulate and monitor wnt signaling in human pluripotent stem cells. Methods in Molecular Biology. Vol. 1481 Humana Press Inc., 2016. pp. 161-181 (Methods in Molecular Biology).
@inbook{e61184f197274aa3ad15fbad8f37395c,
title = "Methods to manipulate and monitor wnt signaling in human pluripotent stem cells",
abstract = "Human pluripotent stem cells (hPSCs) may revolutionize medical practice by providing: (a) a renewable source of cells for tissue replacement therapies, (b) a powerful system to model human diseases in a dish, and (c) a platform for examining efficacy and safety of novel drugs. Furthermore, these cells offer a unique opportunity to study early human development in vitro, in particular, the process by which a seemingly uniform cell population interacts to give rise to the three main embryonic lineages: ectoderm, endoderm. and mesoderm. This process of lineage allocation is regulated by a number of inductive signals that are mediated by growth factors, including FGF, TGFβ, and Wnt. In this book chapter, we introduce a set of tools, methods, and protocols to specifically manipulate the Wnt signaling pathway with the intention of altering the cell fate outcome of hPSCs.",
keywords = "Embryonic development, Human embryonic stem cell (hESC), Human pluripotent stem cell (hPSC), Induced pluripotent stem cell (iPSC), Wnt",
author = "Huggins, {Ian J.} and David Brafman and Karl Willert",
year = "2016",
doi = "10.1007/978-1-4939-6393-5_16",
language = "English (US)",
volume = "1481",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "161--181",
booktitle = "Methods in Molecular Biology",

}

TY - CHAP

T1 - Methods to manipulate and monitor wnt signaling in human pluripotent stem cells

AU - Huggins, Ian J.

AU - Brafman, David

AU - Willert, Karl

PY - 2016

Y1 - 2016

N2 - Human pluripotent stem cells (hPSCs) may revolutionize medical practice by providing: (a) a renewable source of cells for tissue replacement therapies, (b) a powerful system to model human diseases in a dish, and (c) a platform for examining efficacy and safety of novel drugs. Furthermore, these cells offer a unique opportunity to study early human development in vitro, in particular, the process by which a seemingly uniform cell population interacts to give rise to the three main embryonic lineages: ectoderm, endoderm. and mesoderm. This process of lineage allocation is regulated by a number of inductive signals that are mediated by growth factors, including FGF, TGFβ, and Wnt. In this book chapter, we introduce a set of tools, methods, and protocols to specifically manipulate the Wnt signaling pathway with the intention of altering the cell fate outcome of hPSCs.

AB - Human pluripotent stem cells (hPSCs) may revolutionize medical practice by providing: (a) a renewable source of cells for tissue replacement therapies, (b) a powerful system to model human diseases in a dish, and (c) a platform for examining efficacy and safety of novel drugs. Furthermore, these cells offer a unique opportunity to study early human development in vitro, in particular, the process by which a seemingly uniform cell population interacts to give rise to the three main embryonic lineages: ectoderm, endoderm. and mesoderm. This process of lineage allocation is regulated by a number of inductive signals that are mediated by growth factors, including FGF, TGFβ, and Wnt. In this book chapter, we introduce a set of tools, methods, and protocols to specifically manipulate the Wnt signaling pathway with the intention of altering the cell fate outcome of hPSCs.

KW - Embryonic development

KW - Human embryonic stem cell (hESC)

KW - Human pluripotent stem cell (hPSC)

KW - Induced pluripotent stem cell (iPSC)

KW - Wnt

UR - http://www.scopus.com/inward/record.url?scp=84986226605&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84986226605&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-6393-5_16

DO - 10.1007/978-1-4939-6393-5_16

M3 - Chapter

C2 - 27590161

AN - SCOPUS:84986226605

VL - 1481

T3 - Methods in Molecular Biology

SP - 161

EP - 181

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -