Methods and systems for continuous production of Dehalococcoides in a continuous flow stirred-tank reactor

Rosa Krajmalnik-Brown (Inventor), Cesar Torres (Inventor)

Research output: Patent

Abstract

Organic contaminants, such as chlorinated ethenes, are pervasive worldwide, and in situ bioaugmentation is one of the most widely employed methods for remediation. Specialized bacteria of the genus Dehalococcoides are routinely used for in situ bioremediation of chlorinated ethenes and other organic contaminants. For effective remediation it is recommended to have 107 Dehalococcoides cells per liter of groundwater. However, these microbes are slow-growing and difficult to culture in high density. There is only one report where a density of 1012 cells/L of Dehalococcoides was achieved, and it took 35 days to reach that density. Researchers at the Biodesign Institute of Arizona State University have developed a method and system for continuous production of Dehalococcoides in continuous flow stirred-tank reactors (CSTRs). Through optimization of growth conditions, growth medium, and reactor construction materials, a density of 1012 cells/L of Dehalococcoides was achieved in just three days, which is a tenfold improvement over the best reported culture process. This method provides the fastest production rate of high-cell density Dehalococcoides cultures and has the potential to revolutionize production of bioaugmentation cultures for treatment of chlorinated ethenes. Potential Applications High density production of Dehalococcoides in continuous flow stirred-tank reactors Benefits and Advantages Rapid, higher density production - only three days to reach a cell density of 1012 cells/L Cultures can be stored for longer durations - helps streamline production The novel growth medium minimizes proliferation of competing microorganisms and enhances growth of beneficial microbes These Dehalococcoides cultures convert trichloroethene (TCE) to mostly ethene at a 3-d HRT The reactor construction materials minimize possible inhibition of anaerobic microorganisms in the CSTR Dowload Original PDF For more information about the inventor(s) and their research, please see Dr. Krajmalnik-Brown's directory webpage
Original languageEnglish (US)
StatePublished - Feb 1 2013

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ethylene
remediation
microorganism
method
reactor
pollutant
directory
trichloroethylene
bioremediation
bacterium
groundwater
in situ

Cite this

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title = "Methods and systems for continuous production of Dehalococcoides in a continuous flow stirred-tank reactor",
abstract = "Organic contaminants, such as chlorinated ethenes, are pervasive worldwide, and in situ bioaugmentation is one of the most widely employed methods for remediation. Specialized bacteria of the genus Dehalococcoides are routinely used for in situ bioremediation of chlorinated ethenes and other organic contaminants. For effective remediation it is recommended to have 107 Dehalococcoides cells per liter of groundwater. However, these microbes are slow-growing and difficult to culture in high density. There is only one report where a density of 1012 cells/L of Dehalococcoides was achieved, and it took 35 days to reach that density. Researchers at the Biodesign Institute of Arizona State University have developed a method and system for continuous production of Dehalococcoides in continuous flow stirred-tank reactors (CSTRs). Through optimization of growth conditions, growth medium, and reactor construction materials, a density of 1012 cells/L of Dehalococcoides was achieved in just three days, which is a tenfold improvement over the best reported culture process. This method provides the fastest production rate of high-cell density Dehalococcoides cultures and has the potential to revolutionize production of bioaugmentation cultures for treatment of chlorinated ethenes. Potential Applications High density production of Dehalococcoides in continuous flow stirred-tank reactors Benefits and Advantages Rapid, higher density production - only three days to reach a cell density of 1012 cells/L Cultures can be stored for longer durations - helps streamline production The novel growth medium minimizes proliferation of competing microorganisms and enhances growth of beneficial microbes These Dehalococcoides cultures convert trichloroethene (TCE) to mostly ethene at a 3-d HRT The reactor construction materials minimize possible inhibition of anaerobic microorganisms in the CSTR Dowload Original PDF For more information about the inventor(s) and their research, please see Dr. Krajmalnik-Brown's directory webpage",
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year = "2013",
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day = "1",
language = "English (US)",
type = "Patent",

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TY - PAT

T1 - Methods and systems for continuous production of Dehalococcoides in a continuous flow stirred-tank reactor

AU - Krajmalnik-Brown, Rosa

AU - Torres, Cesar

PY - 2013/2/1

Y1 - 2013/2/1

N2 - Organic contaminants, such as chlorinated ethenes, are pervasive worldwide, and in situ bioaugmentation is one of the most widely employed methods for remediation. Specialized bacteria of the genus Dehalococcoides are routinely used for in situ bioremediation of chlorinated ethenes and other organic contaminants. For effective remediation it is recommended to have 107 Dehalococcoides cells per liter of groundwater. However, these microbes are slow-growing and difficult to culture in high density. There is only one report where a density of 1012 cells/L of Dehalococcoides was achieved, and it took 35 days to reach that density. Researchers at the Biodesign Institute of Arizona State University have developed a method and system for continuous production of Dehalococcoides in continuous flow stirred-tank reactors (CSTRs). Through optimization of growth conditions, growth medium, and reactor construction materials, a density of 1012 cells/L of Dehalococcoides was achieved in just three days, which is a tenfold improvement over the best reported culture process. This method provides the fastest production rate of high-cell density Dehalococcoides cultures and has the potential to revolutionize production of bioaugmentation cultures for treatment of chlorinated ethenes. Potential Applications High density production of Dehalococcoides in continuous flow stirred-tank reactors Benefits and Advantages Rapid, higher density production - only three days to reach a cell density of 1012 cells/L Cultures can be stored for longer durations - helps streamline production The novel growth medium minimizes proliferation of competing microorganisms and enhances growth of beneficial microbes These Dehalococcoides cultures convert trichloroethene (TCE) to mostly ethene at a 3-d HRT The reactor construction materials minimize possible inhibition of anaerobic microorganisms in the CSTR Dowload Original PDF For more information about the inventor(s) and their research, please see Dr. Krajmalnik-Brown's directory webpage

AB - Organic contaminants, such as chlorinated ethenes, are pervasive worldwide, and in situ bioaugmentation is one of the most widely employed methods for remediation. Specialized bacteria of the genus Dehalococcoides are routinely used for in situ bioremediation of chlorinated ethenes and other organic contaminants. For effective remediation it is recommended to have 107 Dehalococcoides cells per liter of groundwater. However, these microbes are slow-growing and difficult to culture in high density. There is only one report where a density of 1012 cells/L of Dehalococcoides was achieved, and it took 35 days to reach that density. Researchers at the Biodesign Institute of Arizona State University have developed a method and system for continuous production of Dehalococcoides in continuous flow stirred-tank reactors (CSTRs). Through optimization of growth conditions, growth medium, and reactor construction materials, a density of 1012 cells/L of Dehalococcoides was achieved in just three days, which is a tenfold improvement over the best reported culture process. This method provides the fastest production rate of high-cell density Dehalococcoides cultures and has the potential to revolutionize production of bioaugmentation cultures for treatment of chlorinated ethenes. Potential Applications High density production of Dehalococcoides in continuous flow stirred-tank reactors Benefits and Advantages Rapid, higher density production - only three days to reach a cell density of 1012 cells/L Cultures can be stored for longer durations - helps streamline production The novel growth medium minimizes proliferation of competing microorganisms and enhances growth of beneficial microbes These Dehalococcoides cultures convert trichloroethene (TCE) to mostly ethene at a 3-d HRT The reactor construction materials minimize possible inhibition of anaerobic microorganisms in the CSTR Dowload Original PDF For more information about the inventor(s) and their research, please see Dr. Krajmalnik-Brown's directory webpage

M3 - Patent

ER -