@inbook{ba1de3f8ca074f369d4b84a220a347ee,
title = "Methods and Applications of Campenot Trichamber Neuronal Cultures for the Study of Neuroinvasive Viruses",
abstract = "The development of compartmentalized neuron culture systems has been invaluable in the study of neuroinvasive viruses, including the alpha herpesviruses Herpes Simplex Virus 1 (HSV-1) and Pseudorabies Virus (PRV). This chapter provides updated protocols for assembling and culturing rodent embryonic superior cervical ganglion (SCG) and dorsal root ganglion (DRG) neurons in Campenot trichamber cultures. In addition, we provide several illustrative examples of the types of experiments that are enabled by Campenot cultures: (1) Using fluorescence microscopy to investigate axonal outgrowth/extension through the chambers, and alpha herpesvirus infection, intracellular trafficking, and cell-cell spread via axons. (2) Using correlative fluorescence microscopy and cryo electron tomography to investigate the ultrastructure of virus particles trafficking in axons.",
keywords = "Alphaherpesvirus, Campenot, Cryo electron tomography, Dorsal root ganglia, Fluorescence microscopy, Herpes simplex virus, Neurons, Pseudorabies virus, Superior cervical ganglia, Virus",
author = "Tierney, {Wesley M.} and Vicino, {Ian A.} and Sun, {Stella Y.} and Wah Chiu and Engel, {Esteban A.} and Taylor, {Matthew P.} and Hogue, {Ian B.}",
note = "Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-1990-2_9",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "181--206",
booktitle = "Methods in Molecular Biology",
}