Method for tracking nanogel particles in vivo and in vitro

Brandon L. Seal, Yeong Hau H Lien, Carla Mazar, Mohamad W. Salkini, Tong Cai, Zhibing Hu, Manuel Marquez, Antonio Garcia

Research output: Contribution to journalArticle

Abstract

Hydrogels made of N-isopropylacrylamide (NIPA) can be synthesized in the form of highly monodispersed nanoparticles. After synthesis, NIPA hydrogel nanoparticles (nanogels) can be labeled by Alexa Fluor® 488 carboxylic acid, 2,3,5,6-tetrafluorophenyl ester through amine-terminated functional groups. This choice of dye is complementary to other biological labeling methods for in vivo studies. When the nanogel/clye nanoparticles are injected into rabbits, they can be imaged via tissue sectioning and confocal microscopy, while nanoparticle concentration can be determined by fluorescent microplate assays. Time-course persistence of nanoparticles in the circulatory system can be readily tracked by direct assay of plasma and urine samples using 485 nm excitation and 538 emission wavelengths to keep background fluorescence to nearly the same level as that found using an empty well. Depending upon how the nanoparticles are injected, circulatory system concentrations can reach high concentrations and diminish to low levels or gradually increase and gradually decrease over time. Injection in the femoral artery results in a rapid spike in circulating nanogel/dye concentration, while injection into the renal artery results in a more gradual increase.

Original languageEnglish (US)
Pages (from-to)954-958
Number of pages5
JournalBiotechnology Journal
Volume3
Issue number7
DOIs
StatePublished - Jul 2008

Fingerprint

Nanoparticles
Cardiovascular System
Coloring Agents
Injections
Hydrogels
Hydrogel
Renal Artery
Femoral Artery
Carboxylic Acids
Confocal Microscopy
Amines
In Vitro Techniques
NanoGel
Esters
Fluorescence
Urine
Rabbits
N-isopropylacrylamide

Keywords

  • Fluorescence
  • Microplate
  • Nanogel
  • Nanoparticle
  • NIPA

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Molecular Medicine

Cite this

Seal, B. L., Lien, Y. H. H., Mazar, C., Salkini, M. W., Cai, T., Hu, Z., ... Garcia, A. (2008). Method for tracking nanogel particles in vivo and in vitro. Biotechnology Journal, 3(7), 954-958. https://doi.org/10.1002/biot.200700192

Method for tracking nanogel particles in vivo and in vitro. / Seal, Brandon L.; Lien, Yeong Hau H; Mazar, Carla; Salkini, Mohamad W.; Cai, Tong; Hu, Zhibing; Marquez, Manuel; Garcia, Antonio.

In: Biotechnology Journal, Vol. 3, No. 7, 07.2008, p. 954-958.

Research output: Contribution to journalArticle

Seal, BL, Lien, YHH, Mazar, C, Salkini, MW, Cai, T, Hu, Z, Marquez, M & Garcia, A 2008, 'Method for tracking nanogel particles in vivo and in vitro', Biotechnology Journal, vol. 3, no. 7, pp. 954-958. https://doi.org/10.1002/biot.200700192
Seal BL, Lien YHH, Mazar C, Salkini MW, Cai T, Hu Z et al. Method for tracking nanogel particles in vivo and in vitro. Biotechnology Journal. 2008 Jul;3(7):954-958. https://doi.org/10.1002/biot.200700192
Seal, Brandon L. ; Lien, Yeong Hau H ; Mazar, Carla ; Salkini, Mohamad W. ; Cai, Tong ; Hu, Zhibing ; Marquez, Manuel ; Garcia, Antonio. / Method for tracking nanogel particles in vivo and in vitro. In: Biotechnology Journal. 2008 ; Vol. 3, No. 7. pp. 954-958.
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