Methanosphaerula palustris gen. nov., sp. nov., a hydrogenotrophic methanogen isolated from a minerotrophic fen peatland

Hinsby Cadillo-Quiroz, Joseph B. Yavitt, Stephen H. Zinder

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62 Citations (Scopus)

Abstract

Peatlands are important sources of CH4 emissions to the atmosphere and molecular surveys have identified a diverse, but mainly uncultured, euryarchaeal community in them. Characterization of a strain, E1-9cT, associated with uncultured group E1, from a minerotrophic fen is reported. Cells were regular cocci, usually found in pairs, that stained Gram-positive and were resistant to lysis by 0.1% SDS. Multiple flagella were observed, but motility was not observed in wet mounts. Optimal growth was obtained at moderate temperatures (28-30 °C) and slightly acidic pH (5.5). Total Na+ and NaCl were only tolerated at concentrations less than 100 mM and 0.5 %, respectively, and Na2S concentrations above 0.1 mM were inhibitory. H2/CO2 and formate were the only methanogenic substrates used by E1-9cT; formate concentrations above 50 mM were inhibitory for growth. Vitamins, coenzyme M and acetate (4 mM) were required for growth and the doubling time was about 19 h. Phylogenetic analysis of the 16S rRNA gene and inferred McrA amino acid sequences showed that E1-9cT represented an independent lineage within the order Methanomicrobiales. Physiological and phylogenetic comparisons with different members of the order supported classification of E1-9cT in a new genus in the Methanomicrobiales. The name Methanosphaerula palustris gen. nov., sp. nov. is proposed; strain E1-9cT (=ATCC BAA-1565T =DSM 19958T) is the type strain of Methanosphaerula palustris.

Original languageEnglish (US)
Pages (from-to)928-935
Number of pages8
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume59
Issue number5
DOIs
StatePublished - 2009
Externally publishedYes

Fingerprint

formic acid
methanogens
Methanomicrobiales
fens
fen
peatlands
peatland
formates
new species
Growth
Mesna
phylogenetics
Coccus
Flagella
coenzymes
phylogeny
lysis
motility
vitamin
flagellum

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

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title = "Methanosphaerula palustris gen. nov., sp. nov., a hydrogenotrophic methanogen isolated from a minerotrophic fen peatland",
abstract = "Peatlands are important sources of CH4 emissions to the atmosphere and molecular surveys have identified a diverse, but mainly uncultured, euryarchaeal community in them. Characterization of a strain, E1-9cT, associated with uncultured group E1, from a minerotrophic fen is reported. Cells were regular cocci, usually found in pairs, that stained Gram-positive and were resistant to lysis by 0.1{\%} SDS. Multiple flagella were observed, but motility was not observed in wet mounts. Optimal growth was obtained at moderate temperatures (28-30 °C) and slightly acidic pH (5.5). Total Na+ and NaCl were only tolerated at concentrations less than 100 mM and 0.5 {\%}, respectively, and Na2S concentrations above 0.1 mM were inhibitory. H2/CO2 and formate were the only methanogenic substrates used by E1-9cT; formate concentrations above 50 mM were inhibitory for growth. Vitamins, coenzyme M and acetate (4 mM) were required for growth and the doubling time was about 19 h. Phylogenetic analysis of the 16S rRNA gene and inferred McrA amino acid sequences showed that E1-9cT represented an independent lineage within the order Methanomicrobiales. Physiological and phylogenetic comparisons with different members of the order supported classification of E1-9cT in a new genus in the Methanomicrobiales. The name Methanosphaerula palustris gen. nov., sp. nov. is proposed; strain E1-9cT (=ATCC BAA-1565T =DSM 19958T) is the type strain of Methanosphaerula palustris.",
author = "Hinsby Cadillo-Quiroz and Yavitt, {Joseph B.} and Zinder, {Stephen H.}",
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T1 - Methanosphaerula palustris gen. nov., sp. nov., a hydrogenotrophic methanogen isolated from a minerotrophic fen peatland

AU - Cadillo-Quiroz, Hinsby

AU - Yavitt, Joseph B.

AU - Zinder, Stephen H.

PY - 2009

Y1 - 2009

N2 - Peatlands are important sources of CH4 emissions to the atmosphere and molecular surveys have identified a diverse, but mainly uncultured, euryarchaeal community in them. Characterization of a strain, E1-9cT, associated with uncultured group E1, from a minerotrophic fen is reported. Cells were regular cocci, usually found in pairs, that stained Gram-positive and were resistant to lysis by 0.1% SDS. Multiple flagella were observed, but motility was not observed in wet mounts. Optimal growth was obtained at moderate temperatures (28-30 °C) and slightly acidic pH (5.5). Total Na+ and NaCl were only tolerated at concentrations less than 100 mM and 0.5 %, respectively, and Na2S concentrations above 0.1 mM were inhibitory. H2/CO2 and formate were the only methanogenic substrates used by E1-9cT; formate concentrations above 50 mM were inhibitory for growth. Vitamins, coenzyme M and acetate (4 mM) were required for growth and the doubling time was about 19 h. Phylogenetic analysis of the 16S rRNA gene and inferred McrA amino acid sequences showed that E1-9cT represented an independent lineage within the order Methanomicrobiales. Physiological and phylogenetic comparisons with different members of the order supported classification of E1-9cT in a new genus in the Methanomicrobiales. The name Methanosphaerula palustris gen. nov., sp. nov. is proposed; strain E1-9cT (=ATCC BAA-1565T =DSM 19958T) is the type strain of Methanosphaerula palustris.

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