TY - JOUR
T1 - Methanoregula boonei gen. nov., sp. nov., an acidiphilic methanogen isolated from an acidic peat bog
AU - Bräuer, Suzanna L.
AU - Cadillo-Quiroz, Hinsby
AU - Ward, Rebekah J.
AU - Yavitt, Joseph B.
AU - Zinder, Stephen H.
PY - 2011/1
Y1 - 2011/1
N2 - A novel acidiphilic, hydrogenotrophic methanogen, designated strain 6A8T, was isolated from an acidic (pH 4.0-4.5) and ombrotrophic (rain-fed) bog located near Ithaca, NY, USA. Cultures were dimorphic, containing thin rods (0.2-0.3 μm in diameter and 0.8-3.0 μm long) and irregular cocci (0.2-0.8 μm in diameter). The culture utilized H2/CO 2 to produce methane but did not utilize formate, acetate, methanol, ethanol, 2-propanol, butanol or trimethylamine. Optimal growth conditions were near pH 5.1 and 35 °C. The culture grew in basal medium containing as little as 0.43 mM Na+ and growth was inhibited completely by 50 mM NaCl. To our knowledge, strain 6A8T is one of the most acidiphilic (lowest pH optimum) and salt-sensitive methanogens in pure culture. Acetate, coenzyme M, vitamins and yeast extract were required for growth. It is proposed that a new genus and species be established for this organism, Methanoregula boonei gen. nov., sp. nov. The type strain of Methanoregula boonei is 6A8T (=DSM 21154T =JCM 14090T).
AB - A novel acidiphilic, hydrogenotrophic methanogen, designated strain 6A8T, was isolated from an acidic (pH 4.0-4.5) and ombrotrophic (rain-fed) bog located near Ithaca, NY, USA. Cultures were dimorphic, containing thin rods (0.2-0.3 μm in diameter and 0.8-3.0 μm long) and irregular cocci (0.2-0.8 μm in diameter). The culture utilized H2/CO 2 to produce methane but did not utilize formate, acetate, methanol, ethanol, 2-propanol, butanol or trimethylamine. Optimal growth conditions were near pH 5.1 and 35 °C. The culture grew in basal medium containing as little as 0.43 mM Na+ and growth was inhibited completely by 50 mM NaCl. To our knowledge, strain 6A8T is one of the most acidiphilic (lowest pH optimum) and salt-sensitive methanogens in pure culture. Acetate, coenzyme M, vitamins and yeast extract were required for growth. It is proposed that a new genus and species be established for this organism, Methanoregula boonei gen. nov., sp. nov. The type strain of Methanoregula boonei is 6A8T (=DSM 21154T =JCM 14090T).
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U2 - 10.1099/ijs.0.021782-0
DO - 10.1099/ijs.0.021782-0
M3 - Article
C2 - 20154331
AN - SCOPUS:78651463463
SN - 1466-5026
VL - 61
SP - 45
EP - 52
JO - International Journal of Systematic Bacteriology
JF - International Journal of Systematic Bacteriology
IS - 1
ER -