Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands

Hinsby Cadillo-Quiroz, Suzanna L. Brauer, Noah Goodson, Joseph B. Yavitt, Stephen H. Zinder

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Two mesophilic, hydrogenotrophic methanogens, designated strains SWAN1T and AL-21, were isolated from two contrasting peatlands: a near circumneutral temperate minerotrophic fen in New York State, USA, and an acidic boreal poor fen site in Alaska, USA, respectively. Cells of the two strains were rod-shaped, non-motile, stained Gram-negative and resisted lysis with 0.1% SDS. Cell size was 0.6×1.5-2.8 mm for strain SWAN1T and 0.45-0.85 ×1.5-35 mm for strain AL-21. The strains used H2/CO2 but not formate or other substrates for methanogenesis, grew optimally around 32-37 6C, and their growth spanned through a slightly low to neutral pH range (4.7-7.1). Strain AL-21 grew optimally closer to neutrality at pH 6.2, whereas strain SWAN1T showed a lower optimal pH at 5.4-5.7. The two strains were sensitive to NaCl with a maximal tolerance at 160 mM for strain SWAN1T and 50 mM for strain AL-21. Na2S was toxic at very low concentrations (0.01-0.8 mM), resulting in growth inhibition above these values. The DNA G+C content of the genomes was 35.7 mol% for strain SWAN1T and 35.8 mol% for strain AL-21. Phylogenetic analysis of the 16S rRNA gene sequences showed that the strains are members of the genus Methanobacterium. Strain SWAN1T shared 94-97% similarity with the type strains of recognized species of the genus Methanobacterium, whereas strain AL-21 shared 99% similarity with Methanobacterium lacus 17A1T. On the basis of phenotypic, genomic and phylogenetic characteristics, strain SWAN1T (5DSM 25820T5JCM 18151T) is proposed as the type strain of a novel species, Methanobacterium paludis sp. nov., while strain AL-21 is proposed as a second strain of Methanobacterium lacus.

Original languageEnglish (US)
Pages (from-to)1473-1480
Number of pages8
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume64
Issue numberPART 5
DOIs
StatePublished - 2014

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Methanobacterium
fen
peatlands
Lakes
peatland
phylogenetics
lakes
new species
lysis
methanogenesis
formic acid
genomics
genome
tolerance
DNA
substrate
gene
Poisons
Base Composition
Growth

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands. / Cadillo-Quiroz, Hinsby; Brauer, Suzanna L.; Goodson, Noah; Yavitt, Joseph B.; Zinder, Stephen H.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 64, No. PART 5, 2014, p. 1473-1480.

Research output: Contribution to journalArticle

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title = "Methanobacterium paludis sp. nov. and a novel strain of Methanobacterium lacus isolated from northern peatlands",
abstract = "Two mesophilic, hydrogenotrophic methanogens, designated strains SWAN1T and AL-21, were isolated from two contrasting peatlands: a near circumneutral temperate minerotrophic fen in New York State, USA, and an acidic boreal poor fen site in Alaska, USA, respectively. Cells of the two strains were rod-shaped, non-motile, stained Gram-negative and resisted lysis with 0.1{\%} SDS. Cell size was 0.6×1.5-2.8 mm for strain SWAN1T and 0.45-0.85 ×1.5-35 mm for strain AL-21. The strains used H2/CO2 but not formate or other substrates for methanogenesis, grew optimally around 32-37 6C, and their growth spanned through a slightly low to neutral pH range (4.7-7.1). Strain AL-21 grew optimally closer to neutrality at pH 6.2, whereas strain SWAN1T showed a lower optimal pH at 5.4-5.7. The two strains were sensitive to NaCl with a maximal tolerance at 160 mM for strain SWAN1T and 50 mM for strain AL-21. Na2S was toxic at very low concentrations (0.01-0.8 mM), resulting in growth inhibition above these values. The DNA G+C content of the genomes was 35.7 mol{\%} for strain SWAN1T and 35.8 mol{\%} for strain AL-21. Phylogenetic analysis of the 16S rRNA gene sequences showed that the strains are members of the genus Methanobacterium. Strain SWAN1T shared 94-97{\%} similarity with the type strains of recognized species of the genus Methanobacterium, whereas strain AL-21 shared 99{\%} similarity with Methanobacterium lacus 17A1T. On the basis of phenotypic, genomic and phylogenetic characteristics, strain SWAN1T (5DSM 25820T5JCM 18151T) is proposed as the type strain of a novel species, Methanobacterium paludis sp. nov., while strain AL-21 is proposed as a second strain of Methanobacterium lacus.",
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AU - Yavitt, Joseph B.

AU - Zinder, Stephen H.

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