Metal Ion Requirements and Other Aspects of the Reaction Catalyzed by M1 RNA, the RNA Subunit of Ribonuclease P from Escherichia coli

Cecilia Guerrier-Takada, Karen Haydock, Leland Allen, Sidney Altman

Research output: Contribution to journalArticle

144 Scopus citations

Abstract

M1 RNA, the RNA subunit of ribonuclease P from Escherichia coli, can under certain conditions catalytically cleave precursors to tRNA in the absence of C5, the protein moiety of RNase P. M1 RNA itself is not cleaved during the reaction, nor does it form any covalent bonds with its substrate. Only magnesium and, to a lesser extent, manganese ions can function at the catalytic center of Ml RNA. Several other ions either inhibit the binding of magnesium ion at the active site or function as structural counterions. The reaction rate of cleavage of precursors to tRNAs by M1 RNA is enhanced in the presence of polyethylene glycol) or 2-methyl-2,4-pentanediol. Many aspects of the reaction catalyzed by M1 RNA are compatible with a mechanism in which phosphodiester bond cleavage is mediated by a metal ion.

Original languageEnglish (US)
Pages (from-to)1509-1515
Number of pages7
JournalBiochemistry
Volume25
Issue number7
DOIs
StatePublished - 1986

ASJC Scopus subject areas

  • Biochemistry

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