Messbauer spectroscopy of cyanobacteria Synechocystis sp. PCC 6803 devoid of photosystem I and containing inactive phycobilisomes

A. A. Novakova, L. N. Davletshina, I. V. Elanskaya, A. Yu Aleksandrov, T. Yu Kiseleva, B. K. Semin, I. I. Ivanov, Willem Vermaas, A. B. Rubin

Research output: Contribution to journalArticle

Abstract

Messbauer spectra of the psaAB mutant of Synechocystis sp. PPC 6803 devoid of photosystem I grown in a 57Fe-containing medium were measured. The spectrum is a broadened dublet whose size (about 20%) and parameters (isomeric shift δ = 0.3 mm/s and quadrupole splitting Δ = 0.8 mm/s) suggest the presence of abundant nanoclusters of Fe3+ oxides in a superparamagnetic state tightly bound to the membrane. Treatment of cells with EDTA was accompanied by a substantial (tenfold) decrease in the amount of iron nonspecifically bound to the membrane and the appearance of Fe2+ localized, probably, inside cells and/or cell membranes. In addition, the spectrum of washed cells exhibited superfine magnetic splitting due to iron oxide clusters greater in size than nanoclusters present in the membrane prior to EDTA treatment.

Original languageEnglish (US)
Pages (from-to)484-485
Number of pages2
JournalBiofizika
Volume46
Issue number3
StatePublished - 2001

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Phycobilisomes
Synechocystis
Photosystem I Protein Complex
Cyanobacteria
Spectrum Analysis
Edetic Acid
Membranes
Oxides
Iron
Cell Membrane

Keywords

  • Cyanobacterium
  • Iron
  • Messbauer spectroscopy

ASJC Scopus subject areas

  • Biophysics

Cite this

Novakova, A. A., Davletshina, L. N., Elanskaya, I. V., Aleksandrov, A. Y., Kiseleva, T. Y., Semin, B. K., ... Rubin, A. B. (2001). Messbauer spectroscopy of cyanobacteria Synechocystis sp. PCC 6803 devoid of photosystem I and containing inactive phycobilisomes. Biofizika, 46(3), 484-485.

Messbauer spectroscopy of cyanobacteria Synechocystis sp. PCC 6803 devoid of photosystem I and containing inactive phycobilisomes. / Novakova, A. A.; Davletshina, L. N.; Elanskaya, I. V.; Aleksandrov, A. Yu; Kiseleva, T. Yu; Semin, B. K.; Ivanov, I. I.; Vermaas, Willem; Rubin, A. B.

In: Biofizika, Vol. 46, No. 3, 2001, p. 484-485.

Research output: Contribution to journalArticle

Novakova, AA, Davletshina, LN, Elanskaya, IV, Aleksandrov, AY, Kiseleva, TY, Semin, BK, Ivanov, II, Vermaas, W & Rubin, AB 2001, 'Messbauer spectroscopy of cyanobacteria Synechocystis sp. PCC 6803 devoid of photosystem I and containing inactive phycobilisomes', Biofizika, vol. 46, no. 3, pp. 484-485.
Novakova AA, Davletshina LN, Elanskaya IV, Aleksandrov AY, Kiseleva TY, Semin BK et al. Messbauer spectroscopy of cyanobacteria Synechocystis sp. PCC 6803 devoid of photosystem I and containing inactive phycobilisomes. Biofizika. 2001;46(3):484-485.
Novakova, A. A. ; Davletshina, L. N. ; Elanskaya, I. V. ; Aleksandrov, A. Yu ; Kiseleva, T. Yu ; Semin, B. K. ; Ivanov, I. I. ; Vermaas, Willem ; Rubin, A. B. / Messbauer spectroscopy of cyanobacteria Synechocystis sp. PCC 6803 devoid of photosystem I and containing inactive phycobilisomes. In: Biofizika. 2001 ; Vol. 46, No. 3. pp. 484-485.
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AU - Aleksandrov, A. Yu

AU - Kiseleva, T. Yu

AU - Semin, B. K.

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AU - Vermaas, Willem

AU - Rubin, A. B.

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AB - Messbauer spectra of the psaAB mutant of Synechocystis sp. PPC 6803 devoid of photosystem I grown in a 57Fe-containing medium were measured. The spectrum is a broadened dublet whose size (about 20%) and parameters (isomeric shift δ = 0.3 mm/s and quadrupole splitting Δ = 0.8 mm/s) suggest the presence of abundant nanoclusters of Fe3+ oxides in a superparamagnetic state tightly bound to the membrane. Treatment of cells with EDTA was accompanied by a substantial (tenfold) decrease in the amount of iron nonspecifically bound to the membrane and the appearance of Fe2+ localized, probably, inside cells and/or cell membranes. In addition, the spectrum of washed cells exhibited superfine magnetic splitting due to iron oxide clusters greater in size than nanoclusters present in the membrane prior to EDTA treatment.

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