Abstract
Messbauer spectra of the psaAB mutant of Synechocystis sp. PPC 6803 devoid of photosystem I grown in a 57Fe-containing medium were measured. The spectrum is a broadened dublet whose size (about 20%) and parameters (isomeric shift δ = 0.3 mm/s and quadrupole splitting Δ = 0.8 mm/s) suggest the presence of abundant nanoclusters of Fe3+ oxides in a superparamagnetic state tightly bound to the membrane. Treatment of cells with EDTA was accompanied by a substantial (tenfold) decrease in the amount of iron nonspecifically bound to the membrane and the appearance of Fe2+ localized, probably, inside cells and/or cell membranes. In addition, the spectrum of washed cells exhibited superfine magnetic splitting due to iron oxide clusters greater in size than nanoclusters present in the membrane prior to EDTA treatment.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 484-485 |
| Number of pages | 2 |
| Journal | Biofizika |
| Volume | 46 |
| Issue number | 3 |
| State | Published - 2001 |
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Keywords
- Cyanobacterium
- Iron
- Messbauer spectroscopy
ASJC Scopus subject areas
- Biophysics
Cite this
Messbauer spectroscopy of cyanobacteria Synechocystis sp. PCC 6803 devoid of photosystem I and containing inactive phycobilisomes. / Novakova, A. A.; Davletshina, L. N.; Elanskaya, I. V.; Aleksandrov, A. Yu; Kiseleva, T. Yu; Semin, B. K.; Ivanov, I. I.; Vermaas, Willem; Rubin, A. B.
In: Biofizika, Vol. 46, No. 3, 2001, p. 484-485.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Messbauer spectroscopy of cyanobacteria Synechocystis sp. PCC 6803 devoid of photosystem I and containing inactive phycobilisomes
AU - Novakova, A. A.
AU - Davletshina, L. N.
AU - Elanskaya, I. V.
AU - Aleksandrov, A. Yu
AU - Kiseleva, T. Yu
AU - Semin, B. K.
AU - Ivanov, I. I.
AU - Vermaas, Willem
AU - Rubin, A. B.
PY - 2001
Y1 - 2001
N2 - Messbauer spectra of the psaAB mutant of Synechocystis sp. PPC 6803 devoid of photosystem I grown in a 57Fe-containing medium were measured. The spectrum is a broadened dublet whose size (about 20%) and parameters (isomeric shift δ = 0.3 mm/s and quadrupole splitting Δ = 0.8 mm/s) suggest the presence of abundant nanoclusters of Fe3+ oxides in a superparamagnetic state tightly bound to the membrane. Treatment of cells with EDTA was accompanied by a substantial (tenfold) decrease in the amount of iron nonspecifically bound to the membrane and the appearance of Fe2+ localized, probably, inside cells and/or cell membranes. In addition, the spectrum of washed cells exhibited superfine magnetic splitting due to iron oxide clusters greater in size than nanoclusters present in the membrane prior to EDTA treatment.
AB - Messbauer spectra of the psaAB mutant of Synechocystis sp. PPC 6803 devoid of photosystem I grown in a 57Fe-containing medium were measured. The spectrum is a broadened dublet whose size (about 20%) and parameters (isomeric shift δ = 0.3 mm/s and quadrupole splitting Δ = 0.8 mm/s) suggest the presence of abundant nanoclusters of Fe3+ oxides in a superparamagnetic state tightly bound to the membrane. Treatment of cells with EDTA was accompanied by a substantial (tenfold) decrease in the amount of iron nonspecifically bound to the membrane and the appearance of Fe2+ localized, probably, inside cells and/or cell membranes. In addition, the spectrum of washed cells exhibited superfine magnetic splitting due to iron oxide clusters greater in size than nanoclusters present in the membrane prior to EDTA treatment.
KW - Cyanobacterium
KW - Iron
KW - Messbauer spectroscopy
UR - http://www.scopus.com/inward/record.url?scp=0038970770&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0038970770&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0038970770
VL - 46
SP - 484
EP - 485
JO - Biofizika
JF - Biofizika
SN - 0006-3029
IS - 3
ER -