TY - JOUR
T1 - Membrane depolarization and calcium induce c-fos transcription via phosphorylation of transcription factor CREB
AU - Sheng, Morgan
AU - McFadden, Grant
AU - Greenberg, Michael E.
N1 - Funding Information:
We are indebted to Marc Montminy and Gustav0 Gonzales for a generous gift of anti-CREB antiserum. We are gratefui to Paul Kaplan, Ben Neei, Ann-Bin Shyu, Victor Rivera, Thanos Halazo-netis, and Hunt Potter for their help and expert advice and to Janet Wang and Michael Hwang for technical assistance. This work was supported by Public Health Service grant CA 4385 from the National Institutes of Health. M. E. G. is the recipient of an American Cancer Society Junior Faculty Research Award JFRA-179 and is a Searle Scholar supported by the Chicago Corn-munity Trust. M. S. is supported by a Lucille P. Markey predoctoral fellowship. C. M. is supported by the Alberta Heritage Foundation for Medical Research, Canada.
PY - 1990/4
Y1 - 1990/4
N2 - The mechanism by which the calcium influx signal, triggered by membrane depolarization, is transduced to the nucleus to activate c-fos proto-oncogene transcription has been characterized. A calcium response element (CaRE) that is indistinguishable from a CAMP response element (CRE) mediates transcriptional inducibility by depolarization. Its cognate transcription factor CREB is the target for both calcium and CAMP signals. CREB is rapidly phosphorylated in response to depolarization or cAMP, at a site known to be important for the transcriptional activating function of this protein. The convergent effects of calcium and CAMP on CREB activation are mediated by distinct protein kinase signaling pathways. CREB and its binding site, the Ca/CRE, can thus function as a regulatory element that integrates both calcium and cAMP signals in the control of gene expression.
AB - The mechanism by which the calcium influx signal, triggered by membrane depolarization, is transduced to the nucleus to activate c-fos proto-oncogene transcription has been characterized. A calcium response element (CaRE) that is indistinguishable from a CAMP response element (CRE) mediates transcriptional inducibility by depolarization. Its cognate transcription factor CREB is the target for both calcium and CAMP signals. CREB is rapidly phosphorylated in response to depolarization or cAMP, at a site known to be important for the transcriptional activating function of this protein. The convergent effects of calcium and CAMP on CREB activation are mediated by distinct protein kinase signaling pathways. CREB and its binding site, the Ca/CRE, can thus function as a regulatory element that integrates both calcium and cAMP signals in the control of gene expression.
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U2 - 10.1016/0896-6273(90)90115-V
DO - 10.1016/0896-6273(90)90115-V
M3 - Article
C2 - 2157471
AN - SCOPUS:0025270328
SN - 0896-6273
VL - 4
SP - 571
EP - 582
JO - Neuron
JF - Neuron
IS - 4
ER -