Melanocytes in cultured epithelial grafts are depleted with serial subcultivation and cryopreservation: Implications for clinical outcome

Carolyn Compton, Gretchen Warland, Gunnar Kratz

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Patchy hypopigmentation often occurs unpredictably in the skin regenerated from cultured epidermal autografts, especially when that skin is grown from frozen cells, serially passaged, or both. The impact of serial subcultivation and cryopreservation on melanocyte viability in the cultured epidermal autograft culture system was investigated. Serial subcultivation of human keratinocytes through as many as eight passages was performed, and melanocyte densities in confluent cultures at each passage were determined after specific labeling of melanocytes. The experimental cells were frozen before cultivation and between passages to determine the effect of standard cryopreservation on melanocyte survival. Freshly passaged cells that had not been frozen served as controls. Melanocytes were gradually depleted during fresh passage of epidermal cells but persisted through as many as seven passages. Freezing before or after the first passage or between subsequent passages resulted in a complete loss of melanocytes by the third or fourth passage. The findings suggest that cryopreservation should be avoided during cultured epidermal autograft production to optimize melanocyte survival and minimize pigmentation abnormalities that may occur after grafting.

Original languageEnglish (US)
Pages (from-to)330-336
Number of pages7
JournalJournal of Burn Care and Rehabilitation
Volume19
Issue number4
StatePublished - Jul 1998
Externally publishedYes

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Melanocytes
Cryopreservation
Transplants
Autografts
Hypopigmentation
Skin
Pigmentation
Keratinocytes
Freezing

ASJC Scopus subject areas

  • Rehabilitation
  • Surgery
  • Nursing(all)
  • Health Professions(all)
  • Emergency Medicine

Cite this

Melanocytes in cultured epithelial grafts are depleted with serial subcultivation and cryopreservation : Implications for clinical outcome. / Compton, Carolyn; Warland, Gretchen; Kratz, Gunnar.

In: Journal of Burn Care and Rehabilitation, Vol. 19, No. 4, 07.1998, p. 330-336.

Research output: Contribution to journalArticle

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