Medical bioremediation

a concept moving toward reality.

John Schloendorn, Tim Webb, Kent Kemmish, Mark Hamalainen, David Jackemeyer, Lijing Jiang, Jacques Mathieu, Justin Rebo, Jonathan Sankman, Lindsey Sherman, Lauri Tontson, Ateef Qureshi, Pedro Alvarez, Bruce Rittmann

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Abstract A major driver of aging is catabolic insufficiency, the inability of our bodies to break down certain substances that accumulate slowly throughout the life span. Even though substance buildup is harmless while we are young, by old age the accumulations can reach a toxic threshold and cause disease. This includes some of the most prevalent diseases in old age-atherosclerosis and macular degeneration. Atherosclerosis is associated with the buildup of cholesterol and its oxidized derivatives (particularly 7-ketocholesterol) in the artery wall. Age-related macular degeneration is associated with carotenoid lipofuscin, primarily the pyridinium bisretinoid A2E. Medical bioremediation is the concept of reversing the substance accumulations by using enzymes from foreign species to break down the substances into forms that relieve the disease-related effect. We report on an enzyme discovery project to survey the availability of microorganisms and enzymes with these abilities. We found that such microorganisms and enzymes exist. We identified numerous bacteria having the ability to transform cholesterol and 7-ketocholesterol. Most of these species initiate the breakdown by same reaction mechanism as cholesterol oxidase, and we have used this enzyme directly to reduce the toxicity of 7-ketocholesterol, the major toxic oxysterol, to cultured human cells. We also discovered that soil fungi, plants, and some bacteria possess peroxidase and carotenoid cleavage oxygenase enzymes that effectively destroy with varied degrees of efficiency and selectivity the carotenoid lipofuscin found in macular degeneration.

Original languageEnglish (US)
Pages (from-to)411-419
Number of pages9
JournalRejuvenation Research
Volume12
Issue number6
StatePublished - Dec 2009

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Environmental Biodegradation
Macular Degeneration
Enzymes
Lipofuscin
Poisons
Carotenoids
Atherosclerosis
Cholesterol Oxidase
Cholesterol
Bacteria
Peroxidase
Cultured Cells
Fungi
Soil
Arteries
7-ketocholesterol

ASJC Scopus subject areas

  • Aging
  • Geriatrics and Gerontology

Cite this

Schloendorn, J., Webb, T., Kemmish, K., Hamalainen, M., Jackemeyer, D., Jiang, L., ... Rittmann, B. (2009). Medical bioremediation: a concept moving toward reality. Rejuvenation Research, 12(6), 411-419.

Medical bioremediation : a concept moving toward reality. / Schloendorn, John; Webb, Tim; Kemmish, Kent; Hamalainen, Mark; Jackemeyer, David; Jiang, Lijing; Mathieu, Jacques; Rebo, Justin; Sankman, Jonathan; Sherman, Lindsey; Tontson, Lauri; Qureshi, Ateef; Alvarez, Pedro; Rittmann, Bruce.

In: Rejuvenation Research, Vol. 12, No. 6, 12.2009, p. 411-419.

Research output: Contribution to journalArticle

Schloendorn, J, Webb, T, Kemmish, K, Hamalainen, M, Jackemeyer, D, Jiang, L, Mathieu, J, Rebo, J, Sankman, J, Sherman, L, Tontson, L, Qureshi, A, Alvarez, P & Rittmann, B 2009, 'Medical bioremediation: a concept moving toward reality.', Rejuvenation Research, vol. 12, no. 6, pp. 411-419.
Schloendorn J, Webb T, Kemmish K, Hamalainen M, Jackemeyer D, Jiang L et al. Medical bioremediation: a concept moving toward reality. Rejuvenation Research. 2009 Dec;12(6):411-419.
Schloendorn, John ; Webb, Tim ; Kemmish, Kent ; Hamalainen, Mark ; Jackemeyer, David ; Jiang, Lijing ; Mathieu, Jacques ; Rebo, Justin ; Sankman, Jonathan ; Sherman, Lindsey ; Tontson, Lauri ; Qureshi, Ateef ; Alvarez, Pedro ; Rittmann, Bruce. / Medical bioremediation : a concept moving toward reality. In: Rejuvenation Research. 2009 ; Vol. 12, No. 6. pp. 411-419.
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