Media ion composition controls regulatory and virulence response of Salmonella in spaceflight

James W. Wilson, C. Mark Ott, Laura Quick, Richard Davis, Kerstin Höner zu Bentrup, Aurélie Crabbé, Emily Richter, Shameema Sarker, Jennifer Barrila, Steffen Porwollik, Pui Cheng, Michael McClelland, George Tsaprailis, Timothy Radabaugh, Andrea Hunt, Miti Shah, Mayra Nelman-Gonzalez, Steve Hing, Macarena Parra, Paula DumarsKelly Norwood, Ramona Bober, Jennifer Devich, Ashleigh Ruggles, Autumn CdeBaca, Satro Narayan, Joseph Benjamin, Carla Goulart, Mark Rupert, Luke Catella, Michael J. Schurr, Kent Buchanan, Lisa Morici, James McCracken, Marc D. Porter, Duane L. Pierson, Scott M. Smith, Max Mergeay, Natalie Leys, Heidemarie M. Stefanyshyn-Piper, Dominic Gorie, Cheryl Nickerson

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

The spaceflight environment is relevant to conditions encountered by pathogens during the course of infection and induces novel changes in microbial pathogenesis not observed using conventional methods. It is unclear how microbial cells sense spaceflight-associated changes to their growth environment and orchestrate corresponding changes in molecular and physiological phenotypes relevant to the infection process. Here we report that spaceflight-induced increases in Salmonella virulence are regulated by media ion composition, and that phosphate ion is sufficient to alter related pathogenesis responses in a spaceflight analogue model. Using whole genome microarray and proteomic analyses from two independent. Space Shuttle missions, we identified evolutionary conserved molecular pathways in Salmonella that respond to spaceflight under all media compositions tested. Identification of conserved regulatory paradigms opens new avenues to control microbial responses during the infection process and holds promise to provide an improved understanding of human health and disease on Earth.

Original languageEnglish (US)
Article numbere3923
JournalPLoS One
Volume3
Issue number12
DOIs
StatePublished - Dec 12 2008

Fingerprint

space flight
Space Flight
Salmonella
Virulence
virulence
Ions
ions
Space shuttles
Pathogens
Microarrays
Chemical analysis
Genes
Earth (planet)
Phosphates
Health
pathogenesis
Infection
infection
Microarray Analysis
human diseases

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Media ion composition controls regulatory and virulence response of Salmonella in spaceflight. / Wilson, James W.; Ott, C. Mark; Quick, Laura; Davis, Richard; zu Bentrup, Kerstin Höner; Crabbé, Aurélie; Richter, Emily; Sarker, Shameema; Barrila, Jennifer; Porwollik, Steffen; Cheng, Pui; McClelland, Michael; Tsaprailis, George; Radabaugh, Timothy; Hunt, Andrea; Shah, Miti; Nelman-Gonzalez, Mayra; Hing, Steve; Parra, Macarena; Dumars, Paula; Norwood, Kelly; Bober, Ramona; Devich, Jennifer; Ruggles, Ashleigh; CdeBaca, Autumn; Narayan, Satro; Benjamin, Joseph; Goulart, Carla; Rupert, Mark; Catella, Luke; Schurr, Michael J.; Buchanan, Kent; Morici, Lisa; McCracken, James; Porter, Marc D.; Pierson, Duane L.; Smith, Scott M.; Mergeay, Max; Leys, Natalie; Stefanyshyn-Piper, Heidemarie M.; Gorie, Dominic; Nickerson, Cheryl.

In: PLoS One, Vol. 3, No. 12, e3923, 12.12.2008.

Research output: Contribution to journalArticle

Wilson, JW, Ott, CM, Quick, L, Davis, R, zu Bentrup, KH, Crabbé, A, Richter, E, Sarker, S, Barrila, J, Porwollik, S, Cheng, P, McClelland, M, Tsaprailis, G, Radabaugh, T, Hunt, A, Shah, M, Nelman-Gonzalez, M, Hing, S, Parra, M, Dumars, P, Norwood, K, Bober, R, Devich, J, Ruggles, A, CdeBaca, A, Narayan, S, Benjamin, J, Goulart, C, Rupert, M, Catella, L, Schurr, MJ, Buchanan, K, Morici, L, McCracken, J, Porter, MD, Pierson, DL, Smith, SM, Mergeay, M, Leys, N, Stefanyshyn-Piper, HM, Gorie, D & Nickerson, C 2008, 'Media ion composition controls regulatory and virulence response of Salmonella in spaceflight', PLoS One, vol. 3, no. 12, e3923. https://doi.org/10.1371/journal.pone.0003923
Wilson JW, Ott CM, Quick L, Davis R, zu Bentrup KH, Crabbé A et al. Media ion composition controls regulatory and virulence response of Salmonella in spaceflight. PLoS One. 2008 Dec 12;3(12). e3923. https://doi.org/10.1371/journal.pone.0003923
Wilson, James W. ; Ott, C. Mark ; Quick, Laura ; Davis, Richard ; zu Bentrup, Kerstin Höner ; Crabbé, Aurélie ; Richter, Emily ; Sarker, Shameema ; Barrila, Jennifer ; Porwollik, Steffen ; Cheng, Pui ; McClelland, Michael ; Tsaprailis, George ; Radabaugh, Timothy ; Hunt, Andrea ; Shah, Miti ; Nelman-Gonzalez, Mayra ; Hing, Steve ; Parra, Macarena ; Dumars, Paula ; Norwood, Kelly ; Bober, Ramona ; Devich, Jennifer ; Ruggles, Ashleigh ; CdeBaca, Autumn ; Narayan, Satro ; Benjamin, Joseph ; Goulart, Carla ; Rupert, Mark ; Catella, Luke ; Schurr, Michael J. ; Buchanan, Kent ; Morici, Lisa ; McCracken, James ; Porter, Marc D. ; Pierson, Duane L. ; Smith, Scott M. ; Mergeay, Max ; Leys, Natalie ; Stefanyshyn-Piper, Heidemarie M. ; Gorie, Dominic ; Nickerson, Cheryl. / Media ion composition controls regulatory and virulence response of Salmonella in spaceflight. In: PLoS One. 2008 ; Vol. 3, No. 12.
@article{ba2040ad8d6a419789e0847376c27f86,
title = "Media ion composition controls regulatory and virulence response of Salmonella in spaceflight",
abstract = "The spaceflight environment is relevant to conditions encountered by pathogens during the course of infection and induces novel changes in microbial pathogenesis not observed using conventional methods. It is unclear how microbial cells sense spaceflight-associated changes to their growth environment and orchestrate corresponding changes in molecular and physiological phenotypes relevant to the infection process. Here we report that spaceflight-induced increases in Salmonella virulence are regulated by media ion composition, and that phosphate ion is sufficient to alter related pathogenesis responses in a spaceflight analogue model. Using whole genome microarray and proteomic analyses from two independent. Space Shuttle missions, we identified evolutionary conserved molecular pathways in Salmonella that respond to spaceflight under all media compositions tested. Identification of conserved regulatory paradigms opens new avenues to control microbial responses during the infection process and holds promise to provide an improved understanding of human health and disease on Earth.",
author = "Wilson, {James W.} and Ott, {C. Mark} and Laura Quick and Richard Davis and {zu Bentrup}, {Kerstin H{\"o}ner} and Aur{\'e}lie Crabb{\'e} and Emily Richter and Shameema Sarker and Jennifer Barrila and Steffen Porwollik and Pui Cheng and Michael McClelland and George Tsaprailis and Timothy Radabaugh and Andrea Hunt and Miti Shah and Mayra Nelman-Gonzalez and Steve Hing and Macarena Parra and Paula Dumars and Kelly Norwood and Ramona Bober and Jennifer Devich and Ashleigh Ruggles and Autumn CdeBaca and Satro Narayan and Joseph Benjamin and Carla Goulart and Mark Rupert and Luke Catella and Schurr, {Michael J.} and Kent Buchanan and Lisa Morici and James McCracken and Porter, {Marc D.} and Pierson, {Duane L.} and Smith, {Scott M.} and Max Mergeay and Natalie Leys and Stefanyshyn-Piper, {Heidemarie M.} and Dominic Gorie and Cheryl Nickerson",
year = "2008",
month = "12",
day = "12",
doi = "10.1371/journal.pone.0003923",
language = "English (US)",
volume = "3",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "12",

}

TY - JOUR

T1 - Media ion composition controls regulatory and virulence response of Salmonella in spaceflight

AU - Wilson, James W.

AU - Ott, C. Mark

AU - Quick, Laura

AU - Davis, Richard

AU - zu Bentrup, Kerstin Höner

AU - Crabbé, Aurélie

AU - Richter, Emily

AU - Sarker, Shameema

AU - Barrila, Jennifer

AU - Porwollik, Steffen

AU - Cheng, Pui

AU - McClelland, Michael

AU - Tsaprailis, George

AU - Radabaugh, Timothy

AU - Hunt, Andrea

AU - Shah, Miti

AU - Nelman-Gonzalez, Mayra

AU - Hing, Steve

AU - Parra, Macarena

AU - Dumars, Paula

AU - Norwood, Kelly

AU - Bober, Ramona

AU - Devich, Jennifer

AU - Ruggles, Ashleigh

AU - CdeBaca, Autumn

AU - Narayan, Satro

AU - Benjamin, Joseph

AU - Goulart, Carla

AU - Rupert, Mark

AU - Catella, Luke

AU - Schurr, Michael J.

AU - Buchanan, Kent

AU - Morici, Lisa

AU - McCracken, James

AU - Porter, Marc D.

AU - Pierson, Duane L.

AU - Smith, Scott M.

AU - Mergeay, Max

AU - Leys, Natalie

AU - Stefanyshyn-Piper, Heidemarie M.

AU - Gorie, Dominic

AU - Nickerson, Cheryl

PY - 2008/12/12

Y1 - 2008/12/12

N2 - The spaceflight environment is relevant to conditions encountered by pathogens during the course of infection and induces novel changes in microbial pathogenesis not observed using conventional methods. It is unclear how microbial cells sense spaceflight-associated changes to their growth environment and orchestrate corresponding changes in molecular and physiological phenotypes relevant to the infection process. Here we report that spaceflight-induced increases in Salmonella virulence are regulated by media ion composition, and that phosphate ion is sufficient to alter related pathogenesis responses in a spaceflight analogue model. Using whole genome microarray and proteomic analyses from two independent. Space Shuttle missions, we identified evolutionary conserved molecular pathways in Salmonella that respond to spaceflight under all media compositions tested. Identification of conserved regulatory paradigms opens new avenues to control microbial responses during the infection process and holds promise to provide an improved understanding of human health and disease on Earth.

AB - The spaceflight environment is relevant to conditions encountered by pathogens during the course of infection and induces novel changes in microbial pathogenesis not observed using conventional methods. It is unclear how microbial cells sense spaceflight-associated changes to their growth environment and orchestrate corresponding changes in molecular and physiological phenotypes relevant to the infection process. Here we report that spaceflight-induced increases in Salmonella virulence are regulated by media ion composition, and that phosphate ion is sufficient to alter related pathogenesis responses in a spaceflight analogue model. Using whole genome microarray and proteomic analyses from two independent. Space Shuttle missions, we identified evolutionary conserved molecular pathways in Salmonella that respond to spaceflight under all media compositions tested. Identification of conserved regulatory paradigms opens new avenues to control microbial responses during the infection process and holds promise to provide an improved understanding of human health and disease on Earth.

UR - http://www.scopus.com/inward/record.url?scp=57749177478&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=57749177478&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0003923

DO - 10.1371/journal.pone.0003923

M3 - Article

C2 - 19079590

AN - SCOPUS:57749177478

VL - 3

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 12

M1 - e3923

ER -