Mechanism of interferon action: Studies on the activation of protein phosphorylation and the inhibition of translation in cell-free systems

Bertram Jacobs, N. G. Miyamoto, C. E. Samuel

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We describe the ability of reovirus messenger RNA (mRNA) to serve as a template for translation and as an activator of protein phosphorylation in cell-free extracts prepared from untreated and from interferon (IFN)-treated mouse fibroblast L cells. In vitro transcribed reovirus mRNA was purified by column chromatography on CF-11 cellulose. This procedure removed trace amounts of double-stranded RNA (dsRNA) [0.01%-0.1%] present in mRNA preparations purified solely by extensive LiCl precipitation. In the absence of added dsRNA, CF-11 cellulose-purified reovirus mRNA did not detectably activate phosphorylation of either ribosome-associated protein P1 or the α subunit of protein synthesis initiation factor eIF-2 in S-10 extracts prepared from L cells; the CF-11 cellulose-purified reovirus mRNA was translated more efficiently than was LiCl-purified reovirus mRNA in these extracts. Highly purified CF-11 reovirus mRNA was, however, translated less efficiently by S-10 extracts prepared from IFN-treated L cells than by extracts prepared from untreated L cells, suggesting that the inefficient translation by IFN-treated extracts was an integral property of reovirus mRNA. Increasing the secondary structure of reovirus mRNA by substituting bromouridine (Br-uridine) for uridine in the mRNA caused an increased inhibition of mRNA binding to ribosomes in extracts prepared from IFN-treated as compared to untreated cells. The mechanism of inhibition of translation of CF-11 cellulose-purified reovirus mRNA in IFN-treated systems remains to be established.

Original languageEnglish (US)
Pages (from-to)617-631
Number of pages15
JournalJournal of Interferon Research
Volume8
Issue number5
StatePublished - 1988

Fingerprint

Cell-Free System
Interferons
Phosphorylation
Messenger RNA
Proteins
Cellulose
S 10
Double-Stranded RNA
Cell Extracts
Ribosomes
Prokaryotic Initiation Factor-2
Uridine
Protein Subunits
Chromatography
Fibroblasts

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Mechanism of interferon action : Studies on the activation of protein phosphorylation and the inhibition of translation in cell-free systems. / Jacobs, Bertram; Miyamoto, N. G.; Samuel, C. E.

In: Journal of Interferon Research, Vol. 8, No. 5, 1988, p. 617-631.

Research output: Contribution to journalArticle

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