Measuring nerve growth factor in saliva by immunoassay: A cautionary note

Marla J. Matin; Daming Li; Jon Peterson; Marcus K. Taylor; Heidemarie K. Laurent; Todd Lucas; Steve J. Granger; Douglas A. Granger; Steve W. Granger

doi:10.1016/j.psyneuen.2015.09.030

Measuring nerve growth factor in saliva by immunoassay: A cautionary note

Marla J. Matin, Daming Li, Jon Peterson, Marcus K. Taylor, Heidemarie K. Laurent, Todd Lucas, Steve J. Granger, Douglas A. Granger, Steve W. Granger

Psychology

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Nerve growth factor (NGF), a neurotrophin, modulates a diverse set of physiologic processes in the nervous, immune, and endocrine systems. Studies suggest that NGF can be measured in saliva (sNGF). Historically, the method for measuring sNGF involves the off-label use of an enzyme immunoassay designed for use with cell-culture supernatants/tissue extracts (Nam et al., 2007; Ruhl et al., 2004). In a series of experiments we reveal this measurement strategy is subject to non-specific interference by constituents present in oral fluids. We conclude that the measurement of sNGF by this assay is not optimal for use with oral fluid specimens.

Original language	English (US)
Pages (from-to)	235-237
Number of pages	3
Journal	Psychoneuroendocrinology
Volume	63
DOIs	https://doi.org/10.1016/j.psyneuen.2015.09.030
State	Published - Jan 1 2016

Keywords

Immunoassay
Interference
Methods
Nerve growth factor
Saliva

ASJC Scopus subject areas

Endocrinology, Diabetes and Metabolism
Endocrinology
Endocrine and Autonomic Systems
Psychiatry and Mental health
Biological Psychiatry

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10.1016/j.psyneuen.2015.09.030

Cite this

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title = "Measuring nerve growth factor in saliva by immunoassay: A cautionary note",

abstract = "Nerve growth factor (NGF), a neurotrophin, modulates a diverse set of physiologic processes in the nervous, immune, and endocrine systems. Studies suggest that NGF can be measured in saliva (sNGF). Historically, the method for measuring sNGF involves the off-label use of an enzyme immunoassay designed for use with cell-culture supernatants/tissue extracts (Nam et al., 2007; Ruhl et al., 2004). In a series of experiments we reveal this measurement strategy is subject to non-specific interference by constituents present in oral fluids. We conclude that the measurement of sNGF by this assay is not optimal for use with oral fluid specimens.",

keywords = "Immunoassay, Interference, Methods, Nerve growth factor, Saliva",

author = "Matin, {Marla J.} and Daming Li and Jon Peterson and Taylor, {Marcus K.} and Laurent, {Heidemarie K.} and Todd Lucas and Granger, {Steve J.} and Granger, {Douglas A.} and Granger, {Steve W.}",

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T2 - A cautionary note

AU - Matin, Marla J.

AU - Li, Daming

AU - Peterson, Jon

AU - Taylor, Marcus K.

AU - Laurent, Heidemarie K.

AU - Lucas, Todd

AU - Granger, Steve J.

AU - Granger, Douglas A.

AU - Granger, Steve W.

PY - 2016/1/1

Y1 - 2016/1/1

N2 - Nerve growth factor (NGF), a neurotrophin, modulates a diverse set of physiologic processes in the nervous, immune, and endocrine systems. Studies suggest that NGF can be measured in saliva (sNGF). Historically, the method for measuring sNGF involves the off-label use of an enzyme immunoassay designed for use with cell-culture supernatants/tissue extracts (Nam et al., 2007; Ruhl et al., 2004). In a series of experiments we reveal this measurement strategy is subject to non-specific interference by constituents present in oral fluids. We conclude that the measurement of sNGF by this assay is not optimal for use with oral fluid specimens.

AB - Nerve growth factor (NGF), a neurotrophin, modulates a diverse set of physiologic processes in the nervous, immune, and endocrine systems. Studies suggest that NGF can be measured in saliva (sNGF). Historically, the method for measuring sNGF involves the off-label use of an enzyme immunoassay designed for use with cell-culture supernatants/tissue extracts (Nam et al., 2007; Ruhl et al., 2004). In a series of experiments we reveal this measurement strategy is subject to non-specific interference by constituents present in oral fluids. We conclude that the measurement of sNGF by this assay is not optimal for use with oral fluid specimens.

KW - Immunoassay

KW - Interference

KW - Methods

KW - Nerve growth factor

KW - Saliva

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JO - Psychoneuroendocrinology

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ER -

Measuring nerve growth factor in saliva by immunoassay: A cautionary note

Abstract

Keywords

ASJC Scopus subject areas

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